Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Apr:125:104305.
doi: 10.1016/j.jcv.2020.104305. Epub 2020 Feb 28.

Rapid random access detection of the novel SARS-coronavirus-2 (SARS-CoV-2, previously 2019-nCoV) using an open access protocol for the Panther Fusion

Anne K Cordes  1 , Albert Heim  2
Affiliations

Rapid random access detection of the novel SARS-coronavirus-2 (SARS-CoV-2, previously 2019-nCoV) using an open access protocol for the Panther Fusion

Anne K Cordes et al. J Clin Virol. 2020 Apr.

Abstract

  1. •

    Detection of SARS-CoV-2 within 3.5 h (sample-to-answer-time) by random access real time PCR.

  2. •

    Adaption of an already published real time PCR for SARS-CoV-2 to a fully automatic, random access PCR system.

  3. •

    Detection of all SARS-CoV (from 2002, which may be soon labelled as SARS-CoV-1, and SARS-CoV-2) with a PCR for the highly conserved E gene region.

  4. •

    Differentiation of SARS-CoV-2 with a PCR for the RdRp Gene.

Keywords: 2019-nCoV; COVID-19; Coronavirus; Random access PCR; Rapid diagnosis; SARS-CoV-2.

PubMed Disclaimer

Conflict of interest statement

Declaration of Competing Interest AH: Research grant and travel grant not related to the present work by Hologic Inc. AC: Travel grant and speaker honorarium not related to the present work by Hologic Inc.

References

    1. Chen N., Zhou M., Dong X., Qu J., Gong F., Han Y. Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study. Lancet. 2020;395:507–513. - PMC - PubMed
    1. Zhu N., Zhang D., Wang W., Li X., Yang B., Song J. A novel coronavirus from patients with pneumonia in China, 2019. N. Engl. J. Med. 2020;382:727–733. - PMC - PubMed
    1. Ganzenmueller T., Kaiser R., Baier C., Wehrhane M., Hilfrich B., Witthuhn J. Comparison of the performance of the Panther Fusion respiratory virus panel to R-Gene and laboratory developed tests for diagnostic and hygiene screening specimens from the upper and lower respiratory tract. J. Med. Microbiol. 2020 doi: 10.1099/jmm.0.001133. in press. - DOI - PubMed
    1. Novak-Weekley S.M., Marlowe E.M., Poulter M., Dwyer D., Speers D., Rawlinson W. Evaluation of the Cepheid Xpert Flu Assay for rapid identification and differentiation of influenza A, influenza A 2009 H1N1, and influenza B viruses. J. Clin. Microbiol. 2012;50:1704–1710. - PMC - PubMed
    1. Corman V.M., Landt O., Kaiser M., Molenkamp R., Meijer A., Chu D.K. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020;25 doi: 10.2807/1560-7917.ES.2020.25.3.2000045. - DOI - PMC - PubMed

MeSH terms

Substances