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. 2020 Feb 19:2020:2494128.
doi: 10.1155/2020/2494128. eCollection 2020.

Bone Formation in Grafts with Bio-Oss and Autogenous Bone at Different Proportions in Rabbit Calvaria

Yeon Jung Kim  1 , Carlos Eduardo Takeshi Saiki  1 , Karoline Silva  1 , Carlos Kiyoshi Moreira Massuda  1 , Ana Paula de Souza Faloni  2 , Paulo Henrique Braz-Silva  3   4 , Debora Pallos  1 , Wilson Roberto Sendyk  1
Affiliations

Bone Formation in Grafts with Bio-Oss and Autogenous Bone at Different Proportions in Rabbit Calvaria

Yeon Jung Kim et al. Int J Dent. .

Abstract

Background: The aim of this study was to assess the volumetric stability and bone formation in grafts with Bio-Oss and autogenous bone at different proportions in rabbit calvaria. Material and Methods. Ten rabbits received four titanium cylinders in their calvaria and randomly divided into the following groups: Group I: Bio-Oss (100%), Group II: Bio-Oss (75%) + autogenous bone (25%), Group III: Bio-Oss (50%) + autogenous bone (50%), and Group IV: autogenous bone (100%). After twelve weeks, the animals were euthanized, and samples were collected for clinical and histological analysis.

Results: Clinical analysis showed that Groups I (90.43 ± 8.99) and II (90.87 ± 7.43) had greater dimensional stability compared to Group IV (P=0.0005). Histologically, Groups I, II, and III showed areas of bone formation with particles of biomaterial remaining in close contact with the newly formed bone. However, there were no significant differences between the groups regarding the newly formed bone area.

Conclusion: It was concluded that the use of Bio-Oss either alone or associated with the autogenous bone at a proportion of 25% showed superior dimensional stability compared to the use of autogenous bone in the proposed experimental model.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Schematic drawing of the cylinders distributed into four groups according to the grafts placed in the rabbit caldarium.
Figure 2
Figure 2
Clinical image of the bone grafts in the rabbit calvarium 12 weeks after the surgery.
Figure 3
Figure 3
Photomicrographs showing bone formation in different groups: (a, b) Group I (100% Bio-Oss), (c, d) Group II (75% Bio-Oss + 25% autogenous bone), (e, f) Group III (50% Bio-Oss + 50% autogenous bone), and (g, h, i) Group IV (100% autogenous bone). Hematoxylin and eosin staining of all groups. Bars: 100 μm. Residual particles of biomaterial (B), newly formed bone (NB), bone marrow (BM), autogenous bone graft (AG), osteocyte (arrow), and blood vessels (BV). Extracellular matrix of bone marrow seems to be replaced by adipose tissue (arrowhead). Dotted squares in (a), (c), and (e) represent the areas observed at higher magnification in (b), (d), and (f), respectively. Dotted rectangles (1) and (2) in (g) represent the areas observed at higher magnification in (h) and (i), respectively.
See this image and copyright information in PMC

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