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. 2020 Feb 13:2020:1470868.
doi: 10.1155/2020/1470868. eCollection 2020.

DMBA-Induced Oral Carcinoma in Syrian Hamster: Increased Carcinogenic Effect by Dexamethasone Coexposition

Diana A Martínez B  1 , Paola Andrea Barato Gómez  2 , Carlos Arturo Iregui Castro  3 , Jaiver E Rosas Pérez  1
Affiliations

DMBA-Induced Oral Carcinoma in Syrian Hamster: Increased Carcinogenic Effect by Dexamethasone Coexposition

Diana A Martínez B et al. Biomed Res Int. .

Abstract

Objectives: To investigate the effect of systemic administration of the immunosuppressant dexamethasone (DM) while inducing hamster buccal pouch DMBA carcinogenesis. Materials and Methods. Two different experiments were performed. In the first experiment, hamsters' right buccal pouches in group A (n = 10) were painted three times per week with 7,12-dimethylbenzanthracene (DMBA) 0.5%, while pouches of animals in group B (n = 10) were painted three times per week with 7,12-dimethylbenzanthracene (DMBA) 0.5%, while pouches of animals in group B (.

Results: The time of macroscopic neoplasm development was reduced when DM-DMBA coexposition was employed, finding tumors after 10-12 weeks of exposition. In addition, the frequency of histopathological lesions was higher.

Conclusion: Immunomodulatory action of dexamethasone may reduce the time of oral squamous cell carcinoma (OSCC) induction and may increase the incidence of neoplasms developed.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
Tumor found on the hamster pouch after 14 weeks of DMBA exposition and subsequent s.c. administration of DM 1 mg/kg for seven days. Volume: 14.3 mm3.
Figure 2
Figure 2
Hamsters from the second experiment exposed to DMBA-DM. (a) 4 weeks of exposition (stomatitis). (b) 7 weeks of exposition (fur loss). (c) 10 weeks of exposition (roughness and redness). (d) 13 weeks of exposition (exophytic tumor and papilloma). (e) 15 weeks of exposition (multifocal tumors).
Figure 3
Figure 3
Percentage of animals which presented macroscopic mucosa lesions. Data are shown from week 9 of DMBA exposition. (a) 1st experiment without DM administration. (b) 2nd experiment with DM administration.
Figure 4
Figure 4
Macroscopic and corresponding microscopic images of OSCC development during DM-DMBA concomitant exposition (H&E stained). At 6, dysplasia and hyperkeratosis were evident. Around 12 weeks, papillomas were found macro- and microscopically. Finally, OSCC and multifocal neoplasias were developed on hamsters' pouch.
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References

    1. McGonigle P., Ruggeri B. Animal models of human disease: challenges in enabling translation. Biochemical Pharmacology. 2014;87(1):162–171. doi: 10.1016/J.BCP.2013.08.006. - DOI - PubMed
    1. Curt G. A. The use of animal models in cancer drug discovery and development. Stem Cells. 1994;12(1):23–29. doi: 10.1002/stem.5530120107. - DOI - PubMed
    1. Ishida K., Tomita H., Nakashima T., et al. Current mouse models of oral squamous cell carcinoma: genetic and chemically induced models. Oral Oncology. 2017;73:16–20. doi: 10.1016/J.ORALONCOLOGY.2017.07.028. - DOI - PubMed
    1. Gardner D. Spontaneous squamous cell carcinomas of the oral region in domestic animals: a review and consideration of their relevance to human research. Oral Diseases. 2008;2(2):148–154. doi: 10.1111/j.1601-0825.1996.tb00216.x. - DOI - PubMed
    1. Wong D. T., Todd R., Shklar G., Rustgi A. Head Neck Cancer. Cambridge, MA, USA: Academic Press; 2003. Animal models in head and neck cancer; pp. 57–63.