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. 2020 Feb 18:2020:6247526.
doi: 10.1155/2020/6247526. eCollection 2020.

Human Uterine Decidual NK Cells in Women with a History of Early Pregnancy Enhance Angiogenesis and Trophoblast Invasion

Ningyi Jia  1 , Jian Li  1
Affiliations

Human Uterine Decidual NK Cells in Women with a History of Early Pregnancy Enhance Angiogenesis and Trophoblast Invasion

Ningyi Jia et al. Biomed Res Int. .

Erratum in

Abstract

Objective: The present study aimed to identify changes in decidual natural killer (dNK) cells and related cytokines in women who have undergone induced abortions (IAs). The effects of dNK cells on subsequent pregnancies remain unknown. Accordingly, we sought to investigate whether a history of early pregnancy can change dNK cells and facilitate their role in the regulation of angiogenesis and trophoblast invasion. Materials and Methods. dNK cells were obtained from primiparous women who had undergone IA(s) prior to this study and primiparous women who had never been pregnant before this IA (control). Real-time polymerase chain reaction (PCR) was used to measure the mRNA levels of IFN-γ, IP-10, VEGF, and PLGF in dNK cells. The levels of these cytokines were quantified using the enzyme-linked immunosorbent assay. HUVEC and HTR-8/SVneo cells were used to evaluate the angiogenesis, migration, and invasion activities influenced by dNK cells.

Results: In dNK cells, the mRNA level of IFN-γ, IP-10, VEGF, and PLGF in dNK cells. The levels of these cytokines were quantified using the enzyme-linked immunosorbent assay. HUVEC and HTR-8/SVneo cells were used to evaluate the angiogenesis, migration, and invasion activities influenced by dNK cells.

Conclusion: The findings of this study suggest that a history of early pregnancy has an impact on dNK cells. These trained dNK cells can regulate angiogenesis and trophoblast invasion and migration by promoting the production of certain cytokines.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
Cytokines secretion and mRNA levels in dNK cells of two groups. Cytokines secretion in uterine flushing samples.
Figure 2
Figure 2
Formation by HUVECs of tube structures of two groups. (a, b) Transwell migration showed the impact of dNK cells on HUVEC (c, d).
Figure 3
Figure 3
Transwell migration (a, b) and invasion (c, d) of HTR-8/SVneo of two groups.
See this image and copyright information in PMC

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