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. 2020 Feb 20:2020:7869350.
doi: 10.1155/2020/7869350. eCollection 2020.

The Usefulness of Genotyping of Celiac Disease-Specific HLA among Children with Type 1 Diabetes in Various Clinical Situations

Grazyna Deja  1 , Dominika Sikora  2 , Aleksandra Pyziak-Skupien  1 , Karolina Klenczar  1 , Rafał Deja  3 , Przemysława Jarosz-Chobot  1
Affiliations

The Usefulness of Genotyping of Celiac Disease-Specific HLA among Children with Type 1 Diabetes in Various Clinical Situations

Grazyna Deja et al. J Diabetes Res. .

Abstract

Aim: The aim of the study was to determine the usefulness of HLA DQ2/DQ8 genotyping in children with T1D in various clinical situations: as a screening test at the diabetes onset, as a verification of the diagnosis in doubtful situations, and as a test estimating the risk of CD in the future. Materials and methods. Three groups of patients with T1D were included: newly diagnosed (n = 92), with CD and villous atrophy (n = 92), with CD and villous atrophy (n = 92), with CD and villous atrophy (n = 30), and with potential CD (n = 23). Genetic tests were performed (commercial test, PCR, and REX), and clinical data were collected.

Results: The results of genetic tests confirmed the presence of DQ2/DQ8 in 94% of children with diabetes (group I) and in 100% of children with diabetes and CD (groups II and III, respectively). Comparative analysis of the HLA DQ2/DQ8 distribution did not show any differences. Allele DRB1∗04 (linked with HLA DQ8) was significantly less common in children with diabetes and CD (group I versus groups II and III, 56.5% vs. 24.5%; p = 0.001). The probability of developing CD in DRB1∗04-positive patients was 4 times lower (OR 0.25; 95% CI 0.118-0.529; p = 0.001). The probability of developing CD in DRB1∗04-positive patients was 4 times lower (OR 0.25; 95% CI 0.118-0.529; p = 0.001). The probability of developing CD in DRB1∗04-positive patients was 4 times lower (OR 0.25; 95% CI 0.118-0.529.

Conclusions: Genotyping HLA DQ2/DQ8 as a negative screening has limited use in assessing the risk of CD at the diabetes onset and does not allow to verify the diagnosis of CD in doubtful situations. The presence of the DRB1∗04 allele modulates the risk of CD and significantly reduces it and can predict a potential form.

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Conflict of interest statement

The authors declared that there is no conflict of interests regarding the publication of this paper.

Figures

Figure 1
Figure 1
Distribution of HLA haplotypes in group I (T1D) and groups II and III (T1D and CD); p = 0.321.
Figure 2
Figure 2
(a) ROC curve for age of diabetes diagnosis predisposing for CD (AUC 0.689 (95% CI 0.602, 0.776); p = 0.001). (b) ROC curve for TTG antibody titers predisposing for CD with villous atrophy (AUC 0.744 (95% CI 0.613, 0.876); p = 0.001).
See this image and copyright information in PMC

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