Transcriptome-wide piRNA profiling in human brains for aging genetic factors

Qiao Mao¹, Longhua Fan², Xiaoping Wang³, Xiandong Lin⁴, Yuping Cao⁵, Chengchou Zheng⁶, Yong Zhang⁷, Huihao Zhang⁸, Rolando Garcia-Milian⁹, Longli Kang¹⁰, Jing Shi¹¹, Ting Yu¹¹, Kesheng Wang¹², Lingjun Zuo¹³, Chiang-Shan R Li^{11

13}, Xiaoyun Guo^{13

14}, Xingguang Luo^{11

13}

Affiliations

¹ Department of Psychosomatic Medicine, People's Hospital of Deyang City, Deyang, Sichuan 618000, China.
² Department of Vascular Surgery, Qingpu Branch, Zhongshan Hospital, Fudan University, Shanghai 201700, China.
³ Department of Neurology, Shanghai Tongren Hospital, Shanghai Jiaotong University, Shanghai 200080, China.
⁴ Laboratory of Radiation Oncology and Radiobiology, Fujian Provincial Cancer Hospital, the Teaching Hospital of Fujian Medical University, Fuzhou, Fujian 350014, China.
⁵ Department of Psychiatry, Second Xiangya Hospital, Central South University, Changsha 410012, China.
⁶ Minqing Psychiatric Hospital, Minqing, Fujian 350800, China.
⁷ Tianjin Mental Health Center, Tianjin 300222, China.
⁸ Department of Thyroid and Breast Surgery, The First Affiliated Hospital, Fujian Medical University, Fuzhou 350001, China.
⁹ Curriculum & Research Support Department, Cushing/Whitney Medical Library, Yale University School of Medicine, New Haven, CT 06510, USA.
¹⁰ Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Diseases of Tibet Autonomous Region, Xizang Minzu University School of Medicine, Xiangyang, Shaanxi 712082, China.
¹¹ Biological Psychiatry Research Center, Beijing Huilongguan Hospital, Beijing 100096, China.
¹² Department of Biostatistics and Epidemiology, College of Public Health, East Tennessee State University, Johnson City, TN 37614, USA.
¹³ Department of Psychiatry, Yale University School of Medicine, New Haven, CT 06510, USA.
¹⁴ Shanghai Mental Health Center, Shanghai 200030, China.

PMID: 32149191
PMCID: PMC7059831

Transcriptome-wide piRNA profiling in human brains for aging genetic factors

Qiao Mao et al. Jacobs J Genet. 2019.

. 2019;4(1):014.

Epub 2019 Aug 20.

Authors

Affiliations

¹ Department of Psychosomatic Medicine, People's Hospital of Deyang City, Deyang, Sichuan 618000, China.
² Department of Vascular Surgery, Qingpu Branch, Zhongshan Hospital, Fudan University, Shanghai 201700, China.
³ Department of Neurology, Shanghai Tongren Hospital, Shanghai Jiaotong University, Shanghai 200080, China.
⁴ Laboratory of Radiation Oncology and Radiobiology, Fujian Provincial Cancer Hospital, the Teaching Hospital of Fujian Medical University, Fuzhou, Fujian 350014, China.
⁵ Department of Psychiatry, Second Xiangya Hospital, Central South University, Changsha 410012, China.
⁶ Minqing Psychiatric Hospital, Minqing, Fujian 350800, China.
⁷ Tianjin Mental Health Center, Tianjin 300222, China.
⁸ Department of Thyroid and Breast Surgery, The First Affiliated Hospital, Fujian Medical University, Fuzhou 350001, China.
⁹ Curriculum & Research Support Department, Cushing/Whitney Medical Library, Yale University School of Medicine, New Haven, CT 06510, USA.
¹⁰ Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Diseases of Tibet Autonomous Region, Xizang Minzu University School of Medicine, Xiangyang, Shaanxi 712082, China.
¹¹ Biological Psychiatry Research Center, Beijing Huilongguan Hospital, Beijing 100096, China.
¹² Department of Biostatistics and Epidemiology, College of Public Health, East Tennessee State University, Johnson City, TN 37614, USA.
¹³ Department of Psychiatry, Yale University School of Medicine, New Haven, CT 06510, USA.
¹⁴ Shanghai Mental Health Center, Shanghai 200030, China.

PMID: 32149191
PMCID: PMC7059831

Abstract

Objective: Piwi-interacting RNAs (piRNAs) represent a molecular feature shared by all nonaging biological systems, including the germline and somatic cancer stem cells, which display an indefinite renewal capacity and lifespan-stable genomic integrity and are potentially immortal. Here, we tested the hypothesis that piRNA is a critical genetic determinant of aging in humans.

Methods: Expression of transcriptome-wide piRNAs (n=24k) was profiled in the human prefrontal cortex of 12 subjects (84.9±9.5, range 68-100, years of age) using microarray technology. We examined the correlation between these piRNAs' expression levels and age, adjusting for covariates including disease status.

Results: A total of 9,453 piRNAs were detected in brain. Including seven intergenic and three intronic piRNAs, ten piRNAs were significantly associated with age after correction for multiple testing (|r|=0.9; 1.9×10^-5≤p≤9.9×10^-5).

Conclusion: We conclude that piRNAs might play a potential role in determining the years of survival of humans. The underlying mechanisms might involve the suppression of transposable elements (TEs) and expression regulation of aging-associated genes.

Keywords: Alzheimer’s disease; aging; brain; gene expression; piRNA; transposable elements (TEs); years of survival.

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References

1. Sharma AK, Nelson MC, Brandt JE, et al. Human CD34(+) stem cells express the hiwi gene, a human homologue of the Drosophila gene piwi. Blood. 2001;97(2):426–434. - PubMed
1. Lee EJ, Banerjee S, Zhou H, et al. Identification of piRNAs in the central nervous system. RNA. 2011;17(6):1090–1099. - PMC - PubMed
1. Qiu W, Guo X, Lin X, et al. Transcriptome-wide piRNA profiling in human brains of Alzheimer’s disease. Neurobiology of aging. 2017;57:170–177. - PMC - PubMed
1. Huang CR, Burns KH, Boeke JD. Active transposition in genomes. Annual review of genetics. 2012;46:651–675. - PMC - PubMed
1. Kazazian HH, Jr. Mobile DNA transposition in somatic cells. BMC biology. 2011;9:62. - PMC - PubMed

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Transcriptome-wide piRNA profiling in human brains for aging genetic factors

Affiliations

Transcriptome-wide piRNA profiling in human brains for aging genetic factors

Authors

Affiliations

Abstract

References

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