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. 2020 Dec;11(1):356-374.
doi: 10.1080/21655979.2020.1736238.

Metagenomics for taxonomy profiling: tools and approaches

Mukesh Kumar Awasthi  1   2 , B Ravindran  3 , Surendra Sarsaiya  4 , Hongyu Chen  5 , Steven Wainaina  2 , Ekta Singh  6 , Tao Liu  1 , Sunil Kumar  6 , Ashok Pandey  7 , Lal Singh  6 , Zengqiang Zhang  1
Affiliations

Metagenomics for taxonomy profiling: tools and approaches

Mukesh Kumar Awasthi et al. Bioengineered. 2020 Dec.

Retraction in

Abstract

The study of metagenomics is an emerging field that identifies the total genetic materials in an organism along with the set of all genetic materials like deoxyribonucleic acid and ribose nucleic acid, which play a key role with the maintenance of cellular functions. The best part of this technology is that it gives more flexibility to environmental microbiologists to instantly pioneer the immense genetic variability of microbial communities. However, it is intensively complex to identify the suitable sequencing measures of any specific gene that can exclusively indicate the involvement of microbial metagenomes and be able to advance valuable results about these communities. This review provides an overview of the metagenomic advancement that has been advantageous for aggregation of more knowledge about specific genes, microbial communities and its metabolic pathways. More specific drawbacks of metagenomes technology mainly depend on sequence-based analysis. Therefore, this 'targeted based metagenomics' approach will give comprehensive knowledge about the ecological, evolutionary and functional sequence of significantly important genes that naturally exist in living beings either human, animal and microorganisms from distinctive ecosystems.

Keywords: deoxyribonucleic acid; genomics; metagenomics; microbial metagenomes; ribose nucleic acid.

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Figures

Figure 1.
Figure 1.
General process of metagenomic workflow.
Figure 2.
Figure 2.
Systematic presentation of several environmental factors influencing soil microbial diversity.
Figure 3.
Figure 3.
Systematic presentation of metagenomics.
Figure 4.
Figure 4.
Schematic overview of the flow of resistant microbes (bacteria) and resistance genes in the food supply chain.
Figure 5.
Figure 5.
An overview of biotechnological tools for microbial community analysis.
Figure 6.
Figure 6.
Illustration of potential microbial immigration in (a) waterbodies and distribution system, and (b) wastewater treatment plants.
See this image and copyright information in PMC

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