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. 2020 Feb;56(1):e98.
doi: 10.1002/cpmc.98.

Aseptic Technique

Tomasz Bykowski  1 , Brian Stevenson  2
Affiliations

Aseptic Technique

Tomasz Bykowski et al. Curr Protoc Microbiol. 2020 Feb.

Abstract

This article describes common laboratory procedures that can reduce the risk of culture contamination (sepsis), collectively referred as "aseptic technique." Two major strategies for aseptic work are described: using a Bunsen burner and using a laminar flow hood. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and dispensing aliquots, preparing growth media, and inoculating, passaging, and spreading microorganisms on petri dishes. © 2020 by John Wiley & Sons, Inc.

Keywords: Bunsen burner; aseptic technique; bacteriology; cell culture; laminar flow; laminar hood; sterilization; virology.

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References

LITERATURE CITED

    1. Coico, R., & Lunn, G. (2005). Biosafety: Guidelines for working with pathogenic and infectious microorganisms. Current Protocols in Microbiology, 00, 1A.1.1-1A.1.8. doi: 10.1002/9780471729259.mc01a01s00.
    1. Current Protocols. (2005). Resources for international biosafety guidelines and regulations. Current Protocols in Microbiology, 00, A.1B.1-A.1B.1. doi: 10.1002/9780471729259.mca01bs00.

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