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. 2020 Mar;10(3):131.
doi: 10.1007/s13205-020-2125-6. Epub 2020 Feb 22.

Goat CMTM2: mRNA expression profiles of different alternative spliced variants and associations analyses with growth traits

Libang He #  1 , Zihong Kang #  1 , Yuxin Kang  1 , Weixuan Xiang  1 , Chuanying Pan  1 , Hong Chen  1 , Haijing Zhu  2   3 , Lei Qu  2   3 , Xianyong Lan  1 , Xiaoyue Song  2   3
Affiliations

Goat CMTM2: mRNA expression profiles of different alternative spliced variants and associations analyses with growth traits

Libang He et al. 3 Biotech. 2020 Mar.

Abstract

CKLF like MARVEL transmembrane domain containing 2 (CMTM2) plays crucial roles in spermiogenesis, skeletogenous, growth, and development through PI3K/Akt and other pathways. The purpose of this study was to explore the expression profile and variation of different spliced CMTM2 gene in Shaanbei white cashmere goats, as well as to find the relationships between a CMTM2 promoter region 14 bp genetic variant and growth traits in 1366 Shaanbei white cashmere goats. In this study, we identified alternative CMTM2 splicing and detected the effects of the spliced variants on mRNA expression levels in tissues. Meanwhile, an unreported spliced variant of CMTM2 in goat was identified using in CDS cloning and RT-PCR, namely, CMTM2-AS2. Compared with the normal transcript (CMTM2-AS1), the novel variant had the higher expression level in muscle and liver tissues, indicating that it plays an effective role in growth traits. Furthermore, a 14 bp deletion was detected within CMTM2 promoter region, and the different genotypes were significantly associated with growth traits (e.g., body length, circumference of cannon bone) in the large group of 1366 individuals in Shaanbei white cashmere goats. We found that the body length of the individuals with II (n = 571) genotype had better phenotypes than those with DD (n = 118) and ID (n = 650) genotypes. These results have direct guiding significance for goat breeding in the future and provide a new idea for studying the characteristics and functions of CMTM2 gene in goats.

Keywords: Alternative splicing; Association; CMTM2; Goat; Growth trait; mRNA expression.

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Conflict of interest statement

Conflict of interestWe confirm that this manuscript has not been published in whole or in part and is not being considered for publication elsewhere. There are no any ethical conflicts of interest for all authors. The corresponding authors, Dr. XYS and Dr. XYL take responsibility on behalf of all authors for the authorship, authenticity and integrity of this manuscript, and affirms that all authors and acknowledged contributors have read and approved this manuscript.

Figures

Fig. 1
Fig. 1
Comparison of CMTM2-AS1 and CMTM2-AS2 in goat. a, b The electrophoresis diagrams and the map of structure of goat CMTM2 alternative splicing. c, d The nucleotide and amino acid sequence alignments of CMTM2-AS1 and CMTM2-AS2
Fig. 2
Fig. 2
Tissue expression of CMTM2-AS1 and CMTM2-AS2 in 7d and 42d male goat. Each column denotes the mean ± SE. *P < 0.05
Fig. 3
Fig. 3
The indel locus influence CMTM2-AS1 and CMTM2-AS2 mRNA expression
See this image and copyright information in PMC

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