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. 2020 Jan-Mar;11(1):6-12.
doi: 10.4103/japtr.JAPTR_94_19.

The purity identification and radiolabeling of α-mangostin with technetium-99m

Muchtaridi Muchtaridi  1 , Luthfi Utami Setyawati  1 , Risda Rahmi Islamiaty  1 , Kevin Reinard Lie  1 , Wiwit Nurhidayah  1
Affiliations

The purity identification and radiolabeling of α-mangostin with technetium-99m

Muchtaridi Muchtaridi et al. J Adv Pharm Technol Res. 2020 Jan-Mar.

Abstract

Alpha-mangostin (AM) is a natural compound that has the greatest activity in breast cancer. Radiolabeling AM with technetium-99 m (Tc-99m) has a function as breast cancer radiotracer. This study is aimed to identify the purity of Tc-99m-labeled AM. The identification method was conducted by a validated radio-high-performance liquid chromatography (HPLC) to confirm the chemical purity of the compound when the thin layer of chromatography and paper chromatography were used to find out the radiochemical purity (RCP). The validated radio-HPLC method obtained was C18 column with methanol:water (90:10) as the mobile phase and ultraviolet (243 nm) tandem radioactive detector (Gabi Star). The result showed that the RCP was 70.6% ± 2.87%. The analytical method met the validation criteria according to ICH Q2 (R1); thus, it could be applied in the identification. Unfortunately, the 99mTc-AM identification using radio-HPLC showed that the expected complex was not yet formed perfectly because of chemical impurities.

Keywords: breast cancer; purity identification; 99mTc-alpha-mangostin; radio-high-performance liquid chromatography.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
The radiochemical purity graphics of (a)99mTcO4 (b) technetium-alpha-mangostin (c)99mtechnetium-ethylenediaminetetraacetic acid in paper chromatography
Figure 2
Figure 2
The radiochemical purity graphics of (a)99mTcO4 (b)99mtechnetium-alpha-mangostin (c)99mtechnetium-ethylenediaminetetraacetic acid in thin-layer chromatography paper electrophoresis
Figure 3
Figure 3
The different electrophoresis migration of (a)99mTcO4 (b) technetium-alpha-mangostin (c)99mtechnetium-ethylenediaminetetraacetic acid
Figure 4
Figure 4
High-performance liquid chromatography chromatograms of (a) alpha-mangostin standard (b) blank
Figure 5
Figure 5
High-performance liquid chromatography chromatograms of (a) technetium-alpha-mangostin, (b)99mTc-ethylenediaminetetraacetic acid, (c) alpha-mangostin, (d) ethylenediaminetetraacetic acid, (e) Tc-99m, when the (1) shown the chromatograms from ultraviolet detector and (2) from radioactive detector (Gabi Star)
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