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. 2020 Feb 1;10(1):103-110.
doi: 10.31661/jbpe.v0i0.1254. eCollection 2020 Feb.

Magnetic Resonance Imaging Property of Doxorubicin-Loaded Gadolinium/13X Zeolite/Folic Acid Nanocomposite

Ghaderi S  1   2 , Divband B  3   4 , Gharehaghaji N  5
Affiliations

Magnetic Resonance Imaging Property of Doxorubicin-Loaded Gadolinium/13X Zeolite/Folic Acid Nanocomposite

Ghaderi S et al. J Biomed Phys Eng. .

Abstract

Background: Magnetic resonance imaging (MRI) using nanostructures has been a proper method for tumor targeting purposes. Different MRI nanomaterials, targeting agents and anticancer drugs have been used for targeting of tumors.

Objectives: This study aims to consider the MRI property of doxorubicin (DOX)-loaded gadolinium/13X zeolite/folic acid (Gd3+/13X/FA) nanocomposite.

Material and methods: In this in vitro study, Gd3+/13X/FA/DOX nanocomposite was prepared and the X-ray diffraction, scanning electron microscopy and MTT assay were conducted to evaluate the physicochemical properties of the nanocomposite. MRI was performed at 25°C using a 1.5 T clinical system to determine the T1 relaxation times and subsequently, the T1 relaxivity.

Results: The size of the nanocomposite was in the range of 80-200 nm. The nanocomposite without DOX loading (Gd3+/13X/FA) showed compatibility for A549 cells for all concentrations while DOX-loaded nanocomposite was toxic for 62% of the cells at the concentration of 0.4 mg/ml. The T1 relaxivity of Gd3+/13X/FA/DOX nanocomposite was 4.0401 mM-1s-1.

Conclusion: Gd3+/13X/FA/DOX nanocomposite shows a T1 relaxivity similar to the conventional gadolinium chelates, and a successful DOX loading.

Keywords: 13X zeolite; Doxorubicin; Folic Acid; Gadolinium; Magnetic Resonance Imaging; Relaxivity.

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Figures

Figure1
Figure1
Schematic illustration of Gd3+/13X/FA/DOX nanocomposite.
Figure2
Figure2
a) XRD spectrum and b) SEM image of Gd3+/13X/FA/DOX nanocomposite.
Figure3
Figure3
Relative A549 cell viability following 24 h incubation with various concentrations of Gd3+/13X/FA and Gd3+/13X/FA/DOX nanocomposites.
Figure4
Figure4
a) T1 weighted image of the various Gd3+ concentrations of Gd3+/13X/FA/DOX nanocomposite, b) T1 relaxivity graph of the nanocomposite
See this image and copyright information in PMC

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