New cyclopentenoneacrylic acid derivatives from a marine-derived fungus Trichoderma atroviride H548

Abstract

Three new cyclopentenoneacrylic acid derivatives, trichodermacid A (1), trichodermester A (2), and trichodermester B (3), together with thirteen known compounds, were isolated from an ethyl acetate extract of Trichoderma atroviride H548, a fungus derived from mangrove sediment. The structures of the new compounds were elucidated by spectroscopic methods including HR ESI-MS, ¹H NMR, ¹³C NMR, and 2D-NMR techniques. The antifungal activity of the isolated compounds was evaluated against tea pathogenic fungus Pestalotiopsis theae. Trichodermester A (2) showed potent anti P. theae activity with MIC of 125 μg/disc, while the other compounds were inactive.

Keywords: Trichoderma atroviride H548; antifungal activity; cyclopentenoneacrylic acid derivatives; mangrove sediment.