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. 1988 Aug 19;430(1):73-9.
doi: 10.1016/s0378-4347(00)83135-7.

Determination of (4-chlorophenyl)thiomethylene bisphosphonic acid, a new bisphosphonate, in biological fluids by high-performance liquid chromatography

J P Fels  1 , J Guyonnet, Y Berger, W Cautreels
Affiliations

Determination of (4-chlorophenyl)thiomethylene bisphosphonic acid, a new bisphosphonate, in biological fluids by high-performance liquid chromatography

J P Fels et al. J Chromatogr. .

Abstract

A rapid and sensitive high-performance liquid chromatographic method for the assay of the bisphosphonate (4-chlorophenyl)thiomethylene bisphosphonic acid in plasma and urine is described. It requires selective precipitations and dissolutions of calcium salts prior to reversed-phase chromatography with UV detection. This method used semi-micro scale material and 200-microliters biological aliquots. The limit for accurate quantification is 50 ng/ml. Data on reliability criteria and application to a pharmacokinetic study are presented.

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