Detection of SARS-CoV-2 in Different Types of Clinical Specimens

doi:10.1001/jama.2020.3786

Multicenter Study

. 2020 May 12;323(18):1843-1844.

doi: 10.1001/jama.2020.3786.

Detection of SARS-CoV-2 in Different Types of Clinical Specimens

Wenling Wang¹, Yanli Xu², Ruqin Gao³, Roujian Lu¹, Kai Han², Guizhen Wu¹, Wenjie Tan¹

Affiliations

¹ National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
² Beijing Ditan Hospital, Capital Medical University, Beijing, China.
³ Qingdao Municipal Center for Disease Control and Prevention, Qingdao, China.

PMID: 32159775
PMCID: PMC7066521
DOI: 10.1001/jama.2020.3786

Multicenter Study

Detection of SARS-CoV-2 in Different Types of Clinical Specimens

Wenling Wang et al. JAMA. 2020.

. 2020 May 12;323(18):1843-1844.

doi: 10.1001/jama.2020.3786.

Authors

Wenling Wang¹, Yanli Xu², Ruqin Gao³, Roujian Lu¹, Kai Han², Guizhen Wu¹, Wenjie Tan¹

Affiliations

¹ National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
² Beijing Ditan Hospital, Capital Medical University, Beijing, China.
³ Qingdao Municipal Center for Disease Control and Prevention, Qingdao, China.

PMID: 32159775
PMCID: PMC7066521
DOI: 10.1001/jama.2020.3786

Abstract

This study describes results of PCR and viral RNA testing for SARS-CoV-2 in bronchoalveolar fluid, sputum, feces, blood, and urine specimens from patients with COVID-19 infection in China to identify possible means of non-respiratory transmission.

PubMed Disclaimer

Conflict of interest statement

Conflict of Interest Disclosures: None reported.

Figures

See this image and copyright information in PMC

Comment in

Does nasal screening for Staphylococcus aureus before surgery compromise health care professional safety in the COVID-19 era?
Mayeur N, Berthoumieu P, Charbonneau H. Mayeur N, et al. J Thorac Cardiovasc Surg. 2020 Aug;160(2):e39. doi: 10.1016/j.jtcvs.2020.04.014. Epub 2020 Apr 22. J Thorac Cardiovasc Surg. 2020. PMID: 32448698 Free PMC article. No abstract available.
Expression of Receptors for SARS-CoV-2 in the Gut of Patients with Inflammatory Bowel Disease.
Monteleone G, Franzè E, Laudisi F. Monteleone G, et al. Gut Liver. 2020 Jul 15;14(4):530-531. doi: 10.5009/gnl20112. Gut Liver. 2020. PMID: 32482915 Free PMC article. No abstract available.
Limited role for bronchoalveolar lavage to exclude COVID-19 after negative upper respiratory tract swabs: a multicentre study.
Geri P, Salton F, Zuccatosta L, Tamburrini M, Biolo M, Busca A, Santagiuliana M, Zuccon U, Confalonieri P, Ruaro B, D'Agaro P, Gasparini S, Confalonieri M. Geri P, et al. Eur Respir J. 2020 Oct 8;56(4):2001733. doi: 10.1183/13993003.01733-2020. Print 2020 Oct. Eur Respir J. 2020. PMID: 32764117 Free PMC article.

Cited by

Implementation of the Radiological Society of North America Expert Consensus Guidelines on Reporting Chest CT Findings Related to COVID-19: A Multireader Performance Study.
Som A, Lang M, Yeung T, Carey D, Garrana S, Mendoza DP, Flores EJ, Li MD, Sharma A, McDermott S, Shepard JO, Little BP. Som A, et al. Radiol Cardiothorac Imaging. 2020 Sep 10;2(5):e200276. doi: 10.1148/ryct.2020200276. eCollection 2020 Oct. Radiol Cardiothorac Imaging. 2020. PMID: 33778625 Free PMC article.
Evaluation and comparison of one-step real-time PCR and one-step RT-LAMP methods for detection of SARS-CoV-2.
Hanifehpour H, Ashrafi F, Siasi E, Fallahi S. Hanifehpour H, et al. BMC Infect Dis. 2024 Jul 9;24(1):679. doi: 10.1186/s12879-024-09574-9. BMC Infect Dis. 2024. PMID: 38982392 Free PMC article.
Direct Clinical Evidence Recommending the Use of Proteinase K or Dithiothreitol to Pretreat Sputum for Detection of SARS-CoV-2.
Peng J, Lu Y, Song J, Vallance BA, Jacobson K, Yu HB, Sun Z. Peng J, et al. Front Med (Lausanne). 2020 Sep 18;7:549860. doi: 10.3389/fmed.2020.549860. eCollection 2020. Front Med (Lausanne). 2020. PMID: 33043036 Free PMC article.
All-fiber all-optical quantitative polymerase chain reaction (qPCR).
Li X, Nguyen LV, Hill K, Ebendorff-Heidepriem H, Schartner EP, Zhao Y, Zhou X, Zhang Y, Warren-Smith SC. Li X, et al. Sens Actuators B Chem. 2020 Nov 15;323:128681. doi: 10.1016/j.snb.2020.128681. Epub 2020 Aug 9. Sens Actuators B Chem. 2020. PMID: 32834504 Free PMC article.
Coronavirus Disease 2019 (COVID-19) and Neurosurgery Residency Action Plan: An Institutional Experience from the United States.
Fernandes Cabral DT, Alan N, Agarwal N, Lunsford LD, Monaco EA 3rd. Fernandes Cabral DT, et al. World Neurosurg. 2020 Nov;143:e172-e178. doi: 10.1016/j.wneu.2020.07.080. Epub 2020 Jul 18. World Neurosurg. 2020. PMID: 32693224 Free PMC article.

See all "Cited by" articles

References

1. Tan W, Zhao X, Ma X, et al. . Notes from the field: a novel coronavirus genome identified in a cluster of pneumonia cases—Wuhan, China 2019-2020. China CDC Weekly. 2020;2(4):61-62. - PMC - PubMed
1. Wang D, Hu B, Hu C, et al. . Clinical characteristics of 138 hospitalized patients with 2019 novel coronavirus–infected pneumonia in Wuhan, China. JAMA. Published February 7, 2020. doi:10.1001/jama.2020.1585 - DOI - PMC - PubMed
1. Zhang W, Du RH, Li B, et al. . Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes. Emerg Microbes Infect. 2020;9(1):386-389. doi:10.1080/22221751.2020.1729071 - DOI - PMC - PubMed
1. Pan Y, Zhang D, Yang P, Poon LLM, Wang Q. Viral load of SARS-CoV-2 in clinical samples. Lancet Infect Dis. Published online February 24, 2020. doi:10.1016/S1473-3099(20)30113-4 - DOI - PMC - PubMed

Publication types

Actions
Actions

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

Substances

Actions

LinkOut - more resources

Full Text Sources
Other Literature Sources
- The Lens - Patent Citations Database
Medical
- ClinicalTrials.gov
Miscellaneous
- NCI CPTAC Assay Portal

[1] Tan W, Zhao X, Ma X, et al. . Notes from the field: a novel coronavirus genome identified in a cluster of pneumonia cases—Wuhan, China 2019-2020. China CDC Weekly. 2020;2(4):61-62. - PMC - PubMed

[2] Tan W, Zhao X, Ma X, et al. . Notes from the field: a novel coronavirus genome identified in a cluster of pneumonia cases—Wuhan, China 2019-2020. China CDC Weekly. 2020;2(4):61-62. - PMC - PubMed

[3] Wang D, Hu B, Hu C, et al. . Clinical characteristics of 138 hospitalized patients with 2019 novel coronavirus–infected pneumonia in Wuhan, China. JAMA. Published February 7, 2020. doi:10.1001/jama.2020.1585 - DOI - PMC - PubMed

[4] Wang D, Hu B, Hu C, et al. . Clinical characteristics of 138 hospitalized patients with 2019 novel coronavirus–infected pneumonia in Wuhan, China. JAMA. Published February 7, 2020. doi:10.1001/jama.2020.1585 - DOI - PMC - PubMed

[5] Zhang W, Du RH, Li B, et al. . Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes. Emerg Microbes Infect. 2020;9(1):386-389. doi:10.1080/22221751.2020.1729071 - DOI - PMC - PubMed

[6] Zhang W, Du RH, Li B, et al. . Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes. Emerg Microbes Infect. 2020;9(1):386-389. doi:10.1080/22221751.2020.1729071 - DOI - PMC - PubMed

[7] Pan Y, Zhang D, Yang P, Poon LLM, Wang Q. Viral load of SARS-CoV-2 in clinical samples. Lancet Infect Dis. Published online February 24, 2020. doi:10.1016/S1473-3099(20)30113-4 - DOI - PMC - PubMed

[8] Pan Y, Zhang D, Yang P, Poon LLM, Wang Q. Viral load of SARS-CoV-2 in clinical samples. Lancet Infect Dis. Published online February 24, 2020. doi:10.1016/S1473-3099(20)30113-4 - DOI - PMC - PubMed

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Detection of SARS-CoV-2 in Different Types of Clinical Specimens

Affiliations

Detection of SARS-CoV-2 in Different Types of Clinical Specimens

Authors

Affiliations

Abstract

Conflict of interest statement

Figures

Comment in

Similar articles

Cited by

References

Publication types

MeSH terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Medical

Miscellaneous