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. 2020 Mar;40(2):297-310.
doi: 10.5851/kosfa.2020.e15. Epub 2020 Mar 1.

Probiotic Properties of Lactobacillus brevis KU200019 and Synergistic Activity with Fructooligosaccharides in Antagonistic Activity against Foodborne Pathogens

Kariyawasam Majuwana Gamage Menaka Menike Kariyawasam  1 , Seo Jin Yang  1 , Na-Kyoung Lee  1 , Hyun-Dong Paik  1
Affiliations

Probiotic Properties of Lactobacillus brevis KU200019 and Synergistic Activity with Fructooligosaccharides in Antagonistic Activity against Foodborne Pathogens

Kariyawasam Majuwana Gamage Menaka Menike Kariyawasam et al. Food Sci Anim Resour. 2020 Mar.

Abstract

This study aims to evaluate the probiotic properties of Lactobacillus brevis (L. brevis) KU200019 and the synergistic activity with prebiotics on antimicrobial activity, and the potential application as an adjunct culture in fermented dairy products. The commercial strain, L. brevis ATCC 14869 was used as reference strain. L. brevis KU200019 was showed higher viability in simulated gastric (99.38±0.21%) and bile (115.10±0.13%) conditions compared to reference strain. L. brevis KU200019 exhibited antimicrobial activity against various foodborne pathogens. The supplementation of fructooligosaccharides (FOS) enhanced viability of lactic acid bacteria (>8 Log CFU/mL) and antioxidant activity [2,2-diphenyl-2-picrylhydrazyl radical assay (DPPH) assay, 31.23±1.14%; 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay, 38.82±1.46%] in fermented skim milk during refrigerated storage. L. brevis KU200019 was distinguished from the reference strain by its higher probiotic potential, antimicrobial activity, and higher antioxidant activity in fermented milk. Therefore, L. brevis KU200019 with FOS was demonstrated promising properties for further application in fermented dairy products with enhanced safety and quality.

Keywords: antimicrobial activity; antioxidant activity; dairy application; probiotics; synbiotic.

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Conflict of interest statement

The authors declare no potential conflict of interest.

Figures

Fig. 1.
Fig. 1.. Growth index of Lactobacillus brevis strains after 48 h at 37°C.
The results are the mean of three replicates (mean±SD). Different letters on the top of the bars are significnatly different (p<0.05). Negative control, MRS medium without glucose, FOS, fructooligosachride.
Fig. 2.
Fig. 2.. Adherence of LAB strains to HT-29 cells.
The results are the mean of three replicates (mean±SD). Different letters on the top of the bars are significnatly different (<0.05). LAB, lactic acid bacteria; L. brevis, Lactobacillus brevis; FOS, fructooligosaccharides.
Fig. 3.
Fig. 3.. Adherence of (A) L. monocytogenes (LM) and (B) E. coli (EC) to the HT-29 cells in presence of LAB strains and prebiotics.
The results are the mean of three replicates (mean±SD). Different letters on the top of the bars are significnatly different (p<0.05). LB14869, Lactobacillus brevis ATCC 14869; LB200019, L. brevis KU200019; LAB, lactic acid bacteria; FOS, fructooligosaccharides.
Fig. 4.
Fig. 4.. Bacterial count of (A) Lactobacillus brevis ATCC 14869 and (B) L. brevis KU200019 in skim milk with or without prebiotic compounds during storage at 4°C for 28 days.
The results are the mean of three replicates (mean±SD). FOS, fructooligosaccharides.
Fig. 5.
Fig. 5.. Antioxidant activity of fermented skim milk samples after 7 days.
Different letters on the top of the bars are significnatly different (p<0.05). Fermented skim milk added with C, strater culture; S1, strater culture and Lactobacillus brevis ATCC 14869; S2, strater culture and L. brevis KU200019; S3, strater culture, L. brevis ATCC 14869, and FOS; S4, strater culture, L. brevis KU200019, and FOS. The results are the mean of three replicates (mean±SD). DPPH, 2,2-diphenyl-2-picrylhydrazyl; ABTS, 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid; FOS, fructooligosaccharides.
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