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. 2019 Dec 13;7(23):3925-3929.
doi: 10.3889/oamjms.2019.858. eCollection 2019 Dec 15.

Toxicopathological, Cytogenetic Effects of Acetothioamide on Female Albino Mice Reproductive System

Anas A Humadi  1 , Bushra I Al-Kaisei  2 , Taghreed J Humadai  2 , Ali Ibrahim Ali Al-Ezzy  1
Affiliations

Toxicopathological, Cytogenetic Effects of Acetothioamide on Female Albino Mice Reproductive System

Anas A Humadi et al. Open Access Maced J Med Sci. .

Abstract

Aim: To determine toxicopathological and cytogenetic effects of Acetothioamide (ATA) on the female reproductive system.

Methods: Twenty albino female mice were divided equally into two groups: the first group (control) fed with diet pellet. The second group (treated) were inoculated intraperitoneally with a single dose of ATA (100 mg/kg Bw) for 15 days. All mice were sacrificed at the end of the experiment and blood was collected for evaluation of (FSH and LH), serum peroxy nitrate radical concentration. Cytogenetic analysis (chromosomal aberration, micronuclei, mitotic index and blast index) and the histopathological examination on ovary and uterus were done.

Results: ATA causes significant reduction (p < 0.0001) for FSH, LH and serum peroxy nitrate radical concentration among treated females. Oophoritis, pyometria, thrombosis and endometrial hyperplasia with granulomatous reaction were reported among treated females mainly in uterus tissue and ovary.

Conclusion: ATA causes significant reduction for FSH, LH and serum peroxy nitrate concentration among treated females. Oophoritis, pyometria, thrombosis and endometrial hyperplasia with granulomatous reaction were the main pathological changes in uterus tissue and ovary among treated females.

Keywords: Acetothioamide; Toxicopathological effects; cytogenetic effects; female reproductive system.

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Figures

Figure 1
Figure 1
Histopathological section of the ovary of albino female mice treated with 100 mg/ kg.bw. ATA showed (A) oophoritis characterised by fibrinous exudate (red arrow) mononuclear cells mostly macrophage and lymphocyte and neutrophils (blue arrow), congested blood vessels (yellow arrow), haemorrhage (black arrow), necrosis and apoptosis (white arrow) (H and E stain X40). (B) oophoritis characterised by fibrinous exudate (red arrow) mononuclear cells mostly macrophage and lymphocyte and neutrophils (blue arrow), haemorrhage (black arrow), necrosis and apoptosis (white arrow) (H and E stain X40).
Figure 2
Figure 2
Histopathological section of female uterus mice treated with 100 mg/kg.bw. ATA showed mixed inflammatory mostly macrophage (red arrow), dead neutrophils in the endometrium (black arrow), necrotic area (white arrow) (H and E stain X40).
Figure 3
Figure 3
Histopathological section of female uterus mice treated with 100 mg/kg.bw. ATA showed (A) necrosis (red arrow), (B), free and engulfed hemosiderin pigment by macrophage (yellow arrow) (c) necrosis with polymorphic nuclear cells (black arrow) (H and E stain X40).
Figure 4
Figure 4
Histopathological section of female uterus mice treated with 100 mg/kg.bw. ATA showed (A, B) sever necrotic area of uterus layer (red arrow) endometrium hyperplasia (blue arrow) (C) necrotic area (white arrow), lymphocytic granuloma composed of severing lymphocyte aggregation (yellow arrow) fibrous connective tissue (black arrow) (H and E stain X40)
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