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. 2020 Mar 9:42:11.
doi: 10.1186/s41021-020-00153-3. eCollection 2020.

High-throughput analysis of DNA repair in microplates towards identification of inhibitors

Hiroyuki Kamiya  1
Affiliations

High-throughput analysis of DNA repair in microplates towards identification of inhibitors

Hiroyuki Kamiya. Genes Environ. .

Abstract

Environmental factors can inhibit DNA repair and cause indirect mutagenic actions. Here the author introduces a recent paper on a novel high-throughput system to analyze the various enzymatic activities involved in base excision repair. Such systems will facilitate the identification of compounds that suppressively affect DNA repair.

Keywords: Base excision repair; DNA repair; High-throughput analysis.

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Conflict of interest statement

Competing interestsThe author declares that he has no competing interests.

Figures

Fig. 1
Fig. 1
The principle of the uracil DNA glycosylase assay. The substrate oligodeoxyribonucleotide is fixed to the surface of a microplate and bears a uracil base (shown in red) and fluorescein (shown as F) at the 5′-end. The action of glycosylase a forms an abasic site, and subsequent alkaline denaturation b induces the backbone cleavage and loss of fluorescein
See this image and copyright information in PMC

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References

    1. Margison GP, Santibáñez-Koref MF, Povey AC. Mechanisms of carcinogenicity/chemotherapy by O6-methylguanine. Mutagenesis. 2002;17:483–487. doi: 10.1093/mutage/17.6.483. - DOI - PubMed
    1. Pfeifer GP, You Y-H, Besaratinia A. Mutations induced by ultraviolet light. Mutat Res Fundam Mol Mech Mutagen. 2005;571:19–31. doi: 10.1016/j.mrfmmm.2004.06.057. - DOI - PubMed
    1. Kamiya H. Mutagenic potentials of damaged nucleic acids produced by reactive oxygen/nitrogen species: approaches using synthetic oligonucleotides and nucleotides: survey and summary. Nucleic Acids Res. 2003;31:517–531. doi: 10.1093/nar/gkg137. - DOI - PMC - PubMed
    1. Suzuki T, Kamiya H. Mutations induced by 8-hydroxyguanine (8-oxo-7,8-dihydroguanine), a representative oxidized base, in mammalian cells. Genes Environ. 2017;39:2. doi: 10.1186/s41021-016-0051-y. - DOI - PMC - PubMed
    1. Kawai K, Kasai H, Li Y-S, Kawasaki Y, Watanabe S, Ohta M, Honda T, Yamato H. Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECD. Genes Environ. 2018;40:5. doi: 10.1186/s41021-018-0095-2. - DOI - PMC - PubMed

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