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. 2020 Mar 9:42:11.
doi: 10.1186/s41021-020-00153-3. eCollection 2020.

High-throughput analysis of DNA repair in microplates towards identification of inhibitors

Hiroyuki Kamiya  1
Affiliations

High-throughput analysis of DNA repair in microplates towards identification of inhibitors

Hiroyuki Kamiya. Genes Environ. .

Abstract

Environmental factors can inhibit DNA repair and cause indirect mutagenic actions. Here the author introduces a recent paper on a novel high-throughput system to analyze the various enzymatic activities involved in base excision repair. Such systems will facilitate the identification of compounds that suppressively affect DNA repair.

Keywords: Base excision repair; DNA repair; High-throughput analysis.

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Conflict of interest statement

Competing interestsThe author declares that he has no competing interests.

Figures

Fig. 1
Fig. 1
The principle of the uracil DNA glycosylase assay. The substrate oligodeoxyribonucleotide is fixed to the surface of a microplate and bears a uracil base (shown in red) and fluorescein (shown as F) at the 5′-end. The action of glycosylase a forms an abasic site, and subsequent alkaline denaturation b induces the backbone cleavage and loss of fluorescein
See this image and copyright information in PMC

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