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. 2020 Feb;6(1):49-54.
doi: 10.5114/ceh.2020.93057. Epub 2020 Feb 17.

The effect of curcumin and exercise rehabilitation on liver paraoxonase-1 and NF-kβ gene expression in the rat induced by forced drinking of ethanol

Hoseyn Fatolahi  1 , Mohamad Ali Azarbayjani  1 , Maghsoud Peeri  1 , Hasan Matinhomaei  1
Affiliations

The effect of curcumin and exercise rehabilitation on liver paraoxonase-1 and NF-kβ gene expression in the rat induced by forced drinking of ethanol

Hoseyn Fatolahi et al. Clin Exp Hepatol. 2020 Feb.

Abstract

Aim of the study: Binge ethanol drinking causes liver damage and decreased paraoxonase-1 (PON-1) gene expression. On the other hand, regular physical activity and curcumin consumption as non-invasive interventions can have liver protective effects through enhancing antioxidant defense, and improving PON-1 and NF-kβ (nuclear factor kappa B) gene expression. The aim of this study was to investigate the interactive effect of exercise rehabilitation and curcumin consumption on hepatocyte damage as well as NF-kβ and PON-1 gene expression in rats.

Material and methods: Fifty-six male Wistar rats were randomly selected and equally divided into seven groups: dextrose-control (Dext-Con), ethanol-control (Eth-Con), ethanol-saline (Eth-sal), ethanol-DMSO (Eth-DMSO), ethanol-curcumin (Eth-Cur), ethanol-swimming training (Eth-SWT) and ethanol-SWT + curcumin (Eth-SWT + Cur). After four days of the binge drinking protocol followed by six days of quitting, the interventions of SWT and curcumin (50 mg/kg) were employed for 14 days. Afterwards, the rats' liver tissues were collected and sent to the laboratory for biochemical assays.

Results: The interaction of SWT and curcumin caused an increase in PON-1 gene expression (p = 0.02). In addition, curcumin consumption (p = 0.003) and its interaction with SWT (p = 0.004) resulted in a reduction in NF-kβ gene expression. Also, liver tissue damage was observed in the Eth-Con group compared to other groups.

Conclusions: The combination of curcumin and SWT may be used to reduce the side effects of binge ethanol drinking and improve recovery in the quitting period.

Keywords: alcohol exposure; exercise rehabilitation; herbal medicine; liver damage; turmeric.

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Conflict of interest statement

The authors report no conflict of interest.

Figures

Fig. 1
Fig. 1
Changes in liver PON-1 (A) and NF-kβ (B). *,# Represents significant difference in ethanol-training as well as ethanol-training-curcumin groups compared with ethanol group
Fig. 2
Fig. 2
Histological examination of the liver tissue by Masson’s trichrome staining to evaluate the tissue fibrosis because of increased collagen sedimentation. The collagen sedimentation rate in the tissues of the ethanol group was significantly higher than the dextrose group
Fig. 3
Fig. 3
Histological examination of the liver tissue by hematoxylin and eosin (H&E) staining. Hepatocyte death and high cell spaces were more observed in ethanol groups compared with the Dext-Con group
See this image and copyright information in PMC

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