CRISPR-Cas12b enables efficient plant genome engineering

Meiling Ming^#^{1

2}, Qiurong Ren^#³, Changtian Pan^#¹, Yao He^#³, Yingxiao Zhang^#¹, Shishi Liu³, Zhaohui Zhong³, Jiaheng Wang³, Aimee A Malzahn¹, Jun Wu², Xuelian Zheng³, Yong Zhang⁴, Yiping Qi^{5

6}

Affiliations

¹ Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA.
² Centre of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.
³ Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, China.
⁴ Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, China. zhangyong916@uestc.edu.cn.
⁵ Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA. yiping@umd.edu.
⁶ Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD, USA. yiping@umd.edu.

^# Contributed equally.

PMID: 32170285
DOI: 10.1038/s41477-020-0614-6

CRISPR-Cas12b enables efficient plant genome engineering

Meiling Ming et al. Nat Plants. 2020 Mar.

. 2020 Mar;6(3):202-208.

doi: 10.1038/s41477-020-0614-6. Epub 2020 Mar 9.

Authors

Affiliations

¹ Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA.
² Centre of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.
³ Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, China.
⁴ Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, China. zhangyong916@uestc.edu.cn.
⁵ Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA. yiping@umd.edu.
⁶ Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD, USA. yiping@umd.edu.

^# Contributed equally.

PMID: 32170285
DOI: 10.1038/s41477-020-0614-6

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12b is a newly emerged genome engineering system. Here, we compared Cas12b from Alicyclobacillus acidoterrestris (Aac), Alicyclobacillus acidiphilus (Aa), Bacillus thermoamylovorans (Bth) and Bacillus hisashii (Bh) for genome engineering in rice, an important crop. We found AaCas12b was more efficient than AacCas12b and BthCas12b for targeted mutagenesis, which was further demonstrated in multiplexed genome editing. We also engineered the Cas12b systems for targeted transcriptional repression and activation. Our work establishes Cas12b as the third promising CRISPR system, after Cas9 and Cas12a, for plant genome engineering.

PubMed Disclaimer

References

1. Jinek, M. et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337, 816–821 (2012). - DOI
1. Zetsche, B. et al. Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell 163, 759–771 (2015). - DOI
1. Zhang, Y., Malzahn, A., Sretenovic, S. & Qi, Y. The emerging and uncultivated potential of CRISPR technology in plant science. Nat. Plants 5, 778–791 (2019). - DOI
1. Shmakov, S. et al. Discovery and functional characterization of diverse class 2 CRISPR-Cas systems. Mol. Cell 60, 385–397 (2015). - DOI
1. Teng, F. et al. Repurposing CRISPR-Cas12b for mammalian genome engineering. Cell Discov. 4, 63 (2018). - DOI

Publication types

Actions
Actions

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions

LinkOut - more resources

Other Literature Sources
- The Lens - Patent Citations Database

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

CRISPR-Cas12b enables efficient plant genome engineering

Affiliations

CRISPR-Cas12b enables efficient plant genome engineering

Authors

Affiliations

Abstract

References

Publication types

MeSH terms

LinkOut - more resources

Other Literature Sources