Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Jun;43(12):2459-2466.
doi: 10.1002/jssc.201901242. Epub 2020 Apr 13.

Isolation and purification of alkaloids from the fruits of Macleaya cordata by ionic-liquid-modified high-speed counter-current chromatography

Yuanyuan Liu  1 , Wenjuan Wang  1 , Fenfang Che  1 , Yanzhen Lu  1 , Aoxin Li  1 , Hao Li  2 , Jiangang Liu  2 , Yun Wei  1
Affiliations

Isolation and purification of alkaloids from the fruits of Macleaya cordata by ionic-liquid-modified high-speed counter-current chromatography

Yuanyuan Liu et al. J Sep Sci. 2020 Jun.

Abstract

Macleaya cordata (Willd) R. Br. is a medicinal plant. The most important bioactive compounds of M. cordata are alkaloids that have many biological activities including antifungal, anti-inflammatory, and antitumor. In this study, an ionic-liquid-modified high-speed counter-current chromatography method was established to obtain alkaloids from the fruits of M. cordata. The conditions of ionic-liquid-modified high-speed counter-current chromatography, including solvent systems, the content of ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate [C4 mim][BF4 ]), and the posttreatment of the ionic liquid, were investigated. Five alkaloids protopine, allocryptopine, sanguinarine, 8-O-demethylchelerythrine, and chelerythrine were separated from the extract of the fruits using a high speed counter-current chromatography with two-phase solvent system composed of dichloromethane/methanol/0.3 mol/L hydrochloric acid aqueous solution/[C4 mim][BF4 ] (4:2:2:0.015, v/v). Their purities were 96.33, 95.56, 97.94, 96.22, and 97.90%, respectively. The results indicated that a small amount of ionic liquids as modifier of the two-phase solvent system could shorten the separation time and improve the separation efficiency of the alkaloids from the fruits. The ionic-liquid-modified high-speed counter-current chromatography would provide a feasible way for highly effective separation of alkaloids from natural products.

Keywords: Macleaya cordata (Willd) R. Br; alkaloids; high-speed counter-current chromatography; ionic liquids.

PubMed Disclaimer

References

REFERENCES

    1. Zhang, F., Chen, B., Xiao, S., Yao, S. Z., Optimization and comparison of different extraction techniques for sanguinarine and chelerythrine in fruits of Macleaya cordata (Willd) R. Br. Sep. Purif. Technol. 2005, 42, 283-290.
    1. Liu, Q., Sun, C. L., Meng, F. S., Zhao, W., Li, D. P., Wang, X., Preparative separation of chelerythrine and sanguinarine from Macleaya cordata by pH-zone-refining counter-current chromatography. J. Liq. Chromatogr. 2015, 38, 1789-1793.
    1. Sai, C. M., Li, D. H., Li, S. G., Han, T., Guo, Y. Z., Pei, Y. H., Bai, J., Jing, Y. H., Li, Z. L., Hua, H. M., Racemic alkaloids from Macleaya cordata: structural elucidation, chiral resolution, and cytotoxic, antibacterial activities. Rsc Adv. 2016, 6, 41173-41180.
    1. Ahmad, N., Gupta, S., Husain, M. M., Heiskanen, K. M., Mukhtar, H., Differential antiproliferative and apoptotic response of sanguinarine for cancer cells versus normal cells. Clin. Cancer Res. 2000, 6, 1524-1528.
    1. Zhang, Z. F., Guo, Y., Zhang, L. W., Zhang, J. B., Wei, X. H., Chelerythrine chloride from Macleaya cordata induces growth inhibition and apoptosis in human gastric cancer BGC-823 cells. Acta Pharm. Sin. B. 2012, 2, 464-471.

MeSH terms

LinkOut - more resources