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Review
. 2020 Jun;32(3):191-197.
doi: 10.1097/GCO.0000000000000625.

Oocyte activation, oolemma piercing, and real-time viability confirmation in human oocytes using electrophysiological techniques

Amir Mor  1 , Emre Seli  1   2
Affiliations
Review

Oocyte activation, oolemma piercing, and real-time viability confirmation in human oocytes using electrophysiological techniques

Amir Mor et al. Curr Opin Obstet Gynecol. 2020 Jun.

Abstract

Purpose of review: To discuss the recent applications of electrophysiological principles to the optimization and automation of the IVF laboratory.

Recent findings: There is growing evidence showing improvement of live birth rates following oocyte electro-activation. Novel applications using electrophysiological techniques are now employed to determine oocyte penetration and viability in real-time.

Summary: In this short review, we summarize the recent advances in the integration of electrophysiological techniques into the assisted reproductive technology laboratories. We describe the potential clinical applications and their advantages such as creation of reliable automated cell injection systems and novel manual intracytoplasmic sperm injection (ICSI) training platforms. We also discuss theoretical adverse effects and ways to mitigate them.

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References

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    1. Tosti E, Menezo Y. Gamete activation: basic knowledge and clinical applications. Hum Reprod Update 2016; 22:420–439.
    1. Rubino P, Vigano P, Luddi A, Piomboni P. The ICSI procedure from past to future: a systematic review of the more controversial aspects. Hum Reprod Update 2016; 22:194–227.
    1. Anifandis G, Michopoulos A, Daponte A, et al. Artificial oocyte activation: physiological, pathophysiological and ethical aspects. Syst Biol Reprod Med 2019; 65:3–11.

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