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Review
. 2020 Jun 2;21(11):1573-1581.
doi: 10.1002/cbic.202000058. Epub 2020 Apr 2.

Mechanism and Application of the Catalytic Reaction of [NiFe] Hydrogenase: Recent Developments

Hulin Tai  1 , Shun Hirota  2
Affiliations
Review

Mechanism and Application of the Catalytic Reaction of [NiFe] Hydrogenase: Recent Developments

Hulin Tai et al. Chembiochem. .

Abstract

Hydrogenases (H2 ase) catalyze the oxidation of dihydrogen and the reduction of protons with remarkable efficiency, thereby attracting considerable attention in the energy field due to their biotechnological potential. For this simple reaction, [NiFe] H2 ase has developed a sophisticated but intricate mechanism with the heterolytic cleavage of dihydrogen, where its Ni-Fe active site exhibits various redox states. Recently, new spectroscopic and crystal structure studies of [NiFe] H2 ases have been reported, providing significant insights into the catalytic reaction mechanism, hydrophobic gas-access tunnel, proton-transfer pathway, and electron-transfer pathway of [NiFe] H2 ases. In addition, [NiFe] H2 ases have been shown to play an important role in biofuel cell and solar dihydrogen production. This concept provides an overview of the biocatalytic reaction mechanism and biochemical application of [NiFe] H2 ases based on the new findings.

Keywords: biocatalysis; biofuel cell; hydrogen reaction; hydrogenase; solar fuel.

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References

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