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. 2020 Mar 9;12(3):296.
doi: 10.3390/v12030296.

Vector-Borne Transmission of the Zika Virus Asian Genotype in Europe

Guillaume A Durand  1   2 , Géraldine Piorkowski  2 , Laurence Thirion  2 , Laetitia Ninove  2 , Sandra Giron  3 , Christine Zandotti  2 , Jessica Denis  1   4 , Cyril Badaut  1   4 , Anna-Bella Failloux  5 , Gilda Grard  1   2 , Isabelle Leparc-Goffart  1   2 , Xavier de Lamballerie  2
Affiliations

Vector-Borne Transmission of the Zika Virus Asian Genotype in Europe

Guillaume A Durand et al. Viruses. .

Abstract

Three autochthonous cases of Zika virus occurred in southern France in August 2019. Diagnosis relied on serology and transcription-mediated amplification. Attempts for virus isolation and ZIKV genome RT-PCR detection remained negative. Since the index case was not identified, we addressed the issue of genotyping and geographical origin by performing hemi-nested PCR and sequencing in the Pr gene. Analysis of 16 genotype-specific Single Nucleotides Polymorphisms identified the Asian genotype and suggested a Southeast Asia origin.

Keywords: Aedes albopictus; France; arbovirus; autochthonous transmission; genotyping; zika.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Identification of the genotype (a) and analysis of polymorphism in the Asian genotype (b). * Non-synonymous mutations. Δ Present only in sequence KY288905.
See this image and copyright information in PMC

References

    1. Giron S., Franke F., Decoppet A., Cadiou B., Travaglini T., Thirion L., Durand G., Jeannin C., L’Ambert G., Grard G., et al. Vector-borne transmission of Zika virus in Europe, southern France, August 2019. Euro Surveill. 2019;24:1900655. doi: 10.2807/1560-7917.ES.2019.24.45.1900655. - DOI - PMC - PubMed
    1. Vazeille M., Madec Y., Mousson L., Bellone R., Barré-Cardi H., Sousa C.A., Jiolle D., Yébakima A., de Lamballerie X., Failloux A.-B. Zika virus threshold determines transmission by European Aedes albopictus mosquitoes. Emerg. Microbes Infect. 2019;8:1668–1678. doi: 10.1080/22221751.2019.1689797. - DOI - PMC - PubMed
    1. Pettersson J.H.-O., Bohlin J., Dupont-Rouzeyrol M., Brynildsrud O.B., Alfsnes K., Cao-Lormeau V.-M., Gaunt M.W., Falconar A.K., de Lamballerie X., Eldholm V., et al. Re-visiting the evolution, dispersal and epidemiology of Zika virus in Asia. Emerg. Microbes Infect. 2018;7:79. doi: 10.1038/s41426-018-0082-5. - DOI - PMC - PubMed
    1. Pettersson J.H.-O., Eldholm V., Seligman S.J., Lundkvist Å., Falconar A.K., Gaunt M.W., Musso D., Nougairède A., Charrel R., Gould E.A., et al. How Did Zika Virus Emerge in the Pacific Islands and Latin America? mBio. 2016;7:e01239-16. doi: 10.1128/mBio.01239-16. - DOI - PMC - PubMed
    1. Denis J., Attoumani S., Gravier P., Tenebray B., Garnier A., Briolant S., de Laval F., Chastres V., Grard G., Leparc-Goffart I., et al. High specificity and sensitivity of Zika EDIII-based ELISA diagnosis highlighted by a large human reference panel. PLoS Negl. Trop. Dis. 2019;13:e0007747. doi: 10.1371/journal.pntd.0007747. - DOI - PMC - PubMed