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. 2020 Mar 9;9(3):316.
doi: 10.3390/foods9030316.

Detection of Histamine Based on Gold Nanoparticles with Dual Sensor System of Colorimetric and Fluorescence

Jingran Bi  1   2 , Chuan Tian  1   2 , Gong-Liang Zhang  1   2 , Hongshun Hao  1   2 , Hong-Man Hou  1   2
Affiliations

Detection of Histamine Based on Gold Nanoparticles with Dual Sensor System of Colorimetric and Fluorescence

Jingran Bi et al. Foods. .

Abstract

Gold nanoparticles (Au-NPs), with the dual sensor system of colorimetric and fluorescence responses, were developed for the determination of histamine as a spoilage monitor for distinguishing lifetime and freshness of aquatic products. Upon addition of histamine, the absorption coefficient orders of magnitude via the interaction of free electrons and photons were affected, and the characteristic absorption peak of Au-NPs was red-shifted from 520 nm to 664 nm. Meanwhile, the large amino groups in the networks of histamine-Au-NPs with high molecular orbital exhibited excellent fluorescence behavior at 415 nm. Au-NPs offered a range of 0.001-10.0 μM and 0.01-1.0 μM with a limit of detection of 0.87 nM and 2.04 nM by UV-vis and fluorescence spectrum assay, respectively. Moreover, Au-NPs could be used to semiquantitatively analyze histamine with the naked eye, since the significant colorimetric and fluorescence reaction of Au-NPs solution that coincided with different concentrations of histamine can be observed as the histamine concentration was 0.1-1.0 μM. Both of the dual-sensor systems of Au-NPs were successfully applied to the quantitative analysis of histamine in fresh salmon muscle, suggesting the simplicity and rapidity in the dual detection approaches of Au-NPs might be suitable for spoilage assay of aquatic food to ensure food safety.

Keywords: UV-visible and fluorescence; gold nanoparticles; histamine; spoilage marker; visual detection.

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Conflict of interest statement

All authors declare no conflict of interest.

Figures

Figure 1
Figure 1
FTIR of Gold nanoparticles (Au-NPs) and the aggregation of histamine-Au-NPs.
Figure 2
Figure 2
(A) Average hydrodynamic diameter, polydispersity index (PDI) and zeta potential information, (B) Dynamic Light Scattering (DLS), and (C–D) TEM images of Au-NPs and the aggregation of histamine-Au-NPs.
Scheme 1
Scheme 1
Dual sensing mechanism for determination of histamine by Au-NPs.
Figure 3
Figure 3
(A) UV–vis and (D) fluorescence spectra of Au-NPs with various concentrations of histamine. The corresponding (B) colorimetric and (E) fluorescent response of histamine-Au-NPs under daylight and 365nm UV-light. Linear relationships between (C) the absorption ratios (A664/A520), (F) the fluorescence intensity ratios (I/I0), and the logarithm value of histamine concentrations.
Figure 4
Figure 4
(A) colorimetric, (B) fluorescent response, (C) UV-vis spectra and (D)fluorescence spectra of Au-NPs added with different biological substances containing amino groups (histamine, putrescine, cadaverine, tyramine, phenylethylamine, guanine, guanosine, thymine, inosine, ATP, and AMP) at concentrations of 1.0 μM.
See this image and copyright information in PMC

References

    1. Heerthana V.R., Preetha R. Biosensors: A potential tool for quality assurance and food safety pertaining to biogenic amines/volatile amines formation in aquaculture systems/products. Rev. Aquacult. 2019;11:220–233. doi: 10.1111/raq.12236. - DOI
    1. Sørensen K.M., Aru V., Khakimov B., Aunskjær U., Engelsen S.B. Biogenic Amines: A key freshness parameter of animal protein products in the coming circular economy. Curr. Opin. Food Sci. 2018;22:167–173. doi: 10.1016/j.cofs.2018.05.014. - DOI
    1. Del R.B., Redruello B., Linares D.M., Ladero V., Fernandez M., Martin M.C., Ruas-Madiedo P., Alvarez M.A. The dietary biogenic amines tyramine and histamine show synergistic toxicity towards intestinal cells in culture. Food Chem. 2017;218:249–255. doi: 10.1016/j.foodchem.2016.09.046. - DOI - PubMed
    1. Yang H., Yoon M., Um M.Y., Lee J., Jung J., Lee C., Kim Y.T., Kwon S., Kim B., Cho S. Sleep-Promoting Effects and Possible Mechanisms of Action Associated with a Standardized Rice Bran Supplement. Nutrients. 2017;9:512. doi: 10.3390/nu9050512. - DOI - PMC - PubMed
    1. Altieri I., Semeraro A., Scalise F., Calderari I., Stacchini P. European official control of food: Determination of histamine in fish products by a HPLC–UV-DAD method. Food Chem. 2016;211:694–699. doi: 10.1016/j.foodchem.2016.05.111. - DOI - PubMed

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