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. 2020 Mar 14;10(3):451.
doi: 10.3390/biom10030451.

Polyphenolic Compounds from Lespedeza Bicolor Root Bark Inhibit Progression of Human Prostate Cancer Cells via Induction of Apoptosis and Cell Cycle Arrest

Sergey A Dyshlovoy  1   2   3 , Darya Tarbeeva  4 , Sergey Fedoreyev  4 , Tobias Busenbender  1 , Moritz Kaune  1 , Marina Veselova  4 , Anatoliy Kalinovskiy  4 , Jessica Hauschild  1 , Valeria Grigorchuk  5 , Natalya Kim  4 , Carsten Bokemeyer  1 , Markus Graefen  3 , Petr Gorovoy  4 , Gunhild von Amsberg  1   3
Affiliations

Polyphenolic Compounds from Lespedeza Bicolor Root Bark Inhibit Progression of Human Prostate Cancer Cells via Induction of Apoptosis and Cell Cycle Arrest

Sergey A Dyshlovoy et al. Biomolecules. .

Abstract

From a root bark of Lespedeza bicolor Turch we isolated two new (7 and 8) and six previously known compounds (1-6) belonging to the group of prenylated polyphenols. Their structures were elucidated using mass spectrometry, nuclear magnetic resonance and circular dichroism spectroscopy. These natural compounds selectively inhibited human drug-resistant prostate cancer in vitro. Prenylated pterocarpans 1-3 prevented the cell cycle progression of human cancer cells in S-phase. This was accompanied by a reduced expression of mRNA corresponding to several human cyclin-dependent kinases (CDKs). In contrast, compounds 4-8 induced a G1-phase cell cycle arrest without any pronounced effect on CDKs mRNA expression. Interestingly, a non-substituted hydroxy group at C-8 of ring D of the pterocarpan skeleton of compounds 1-3 seems to be important for the CDKs inhibitory activity.

Keywords: Lespedeza bicolor; Polyphenolic compounds; apoptosis; cell cycle; prostate cancer; pterocarpans.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The structures of polyphenolic compounds 1–8 isolated from L. bicolor root bark and stem bark.
Figure 1
Figure 1
The structures of polyphenolic compounds 1–8 isolated from L. bicolor root bark and stem bark.
Figure 2
Figure 2
HPLC profiles of L. bicolor stem bark (A) and root bark (B) ethanolic extracts.
Figure 2
Figure 2
HPLC profiles of L. bicolor stem bark (A) and root bark (B) ethanolic extracts.
Figure 3
Figure 3
Effect of natural compounds on DNA fragmentation of PC-3 cells. Cells were treated with the investigated compounds for 24 h. The effects were analyzed by flow cytometry. Cells detected as sub-G1 population were considered to be apoptotic. Cisplatin (Cis) was used as a positive control. * p ≤ 0.05 (Student’s t-test).
Figure 4
Figure 4
Treatment effects on the progression of the cell cycle of PC-3 cells. Cells were treated with the investigated compounds for 24 h. The effects were analyzed by flow cytometry. Cisplatin was used as a positive control. * p ≤ 0.05 (Student’s t-test).
Figure 5
Figure 5
Expression of mRNA correspondent to CDK1-9 in PC-3 cells treated with the isolated compounds 1–8 for 24 h. The target gene expression was normalized to GAPDH gene expression. * p ≤ 0.05 (Student’s t-test).
See this image and copyright information in PMC

References

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