Analyzing electron transport chain supercomplexes

Abstract

This review focuses on three independent and complementary approaches to obtain information on the combined function of respiratory complexes when present in different structural situations, either as individual complexes or when superassembled with other complexes. We review the utility of in-gel activity after blue native electrophoresis, integrated oxygen consumption of supercomplexes containing complex IV, and spectrophotometric activity measurements.

Keywords: Blue native gel electrophoresis; Electron transport chain; Mitochondria respiration; Oxidative phosphorylation; Supercomplexes.