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. 2020 Jul;140(1):95-97.
doi: 10.1007/s00401-020-02149-3. Epub 2020 Mar 17.

Genome-wide DNA methylation profiles distinguish silent from non-silent ACTH adenomas

Franz L Ricklefs  1 , Krystian D Fita  1 , Roman Rotermund  1 , Andras Piffko  1 , Simone Schmid  2 , David Capper  2 , Rolf Buslei  3 , Michael Buchfelder  4 , Till Burkhardt  5 , Jakob Matschke  6 , Katrin Lamszus  1 , Manfred Westphal  1 , Ulrich Schüller  7   8   9 , Jörg Flitsch  10
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Genome-wide DNA methylation profiles distinguish silent from non-silent ACTH adenomas

Franz L Ricklefs et al. Acta Neuropathol. 2020 Jul.
No abstract available

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig.1
Fig.1
Genome-wide DNA methylation profiles split ACTH adenomas into clinically relevant subgroups. a Corticotroph adenomas causing either Cushing’s disease (CD) or being clinically silent (SCA) show strong expression of adrenocorticotrophic hormone (ACTH). Scale bar: 100 µm. b SCA patients have larger tumors in comparison to CD as measured by MRI (p < 0.0001). c Cumulative copy number alterations (CNA) inferred from the DNA methylation data. Healthy pituitary gland samples derived from the publicly accessible Capper set [2] were used as controls. d Heat map using pairwise Pearson correlation coefficients of the 10,000 most variable CpG features across all 36 samples reveals two distinct CD and SCA clusters and a third group with a clinically heterogeneous picture. No AL = no anterior lobe. eT-Distributed Stochastic Neighbor Embedding (t-SNE) of all methylation probes indicates that SCA and CDs are distinguishable by their epigenetic profiles. fT-SNE analysis with all 36 ACTH adenomas merged with the CNS tumor reference cohort from Capper et al. (2018).
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References

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