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. 2020 Jul;114(1):127-139.
doi: 10.1111/mmi.14500. Epub 2020 Apr 10.

Methylation-dependent transcriptional regulation of crescentin gene (creS) by GcrA in Caulobacter crescentus

Saswat S Mohapatra  1 , Antonella Fioravanti  1 , Pauline Vandame  1 , Corentin Spriet  1 , Francesco Pini  1 , Coralie Bompard  1 , Ralf Blossey  1 , Odile Valette  2 , Emanuele G Biondi  2
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Free article

Methylation-dependent transcriptional regulation of crescentin gene (creS) by GcrA in Caulobacter crescentus

Saswat S Mohapatra et al. Mol Microbiol. 2020 Jul.
Free article

Abstract

In Caulobacter crescentus the combined action of chromosome replication and the expression of DNA methyl-transferase CcrM at the end of S-phase maintains a cyclic alternation between a full- to hemi-methylated chromosome. This transition of the chromosomal methylation pattern affects the DNA-binding properties of the transcription factor GcrA that controls the several key cell cycle functions. However, the molecular mechanism by which GcrA and methylation are linked to transcription is not fully elucidated yet. Using a combination of cell biology, genetics, and in vitro analysis, we deciphered how GcrA integrates the methylation pattern of several S-phase expressed genes to their transcriptional output. We demonstrated in vitro that transcription of ctrA from the P1 promoter in its hemi-methylated state is activated by GcrA, while in its fully methylated state GcrA had no effect. Further, GcrA and methylation together influence a peculiar distribution of creS transcripts, encoding for crescentin, the protein responsible for the characteristic shape of Caulobacter cells. This gene is duplicated at the onset of chromosome replication and the two hemi-methylated copies are spatially segregated. Our results indicated that GcrA transcribed only the copy where coding strand is methylated. In vitro transcription assay further substantiated this finding. As several of the cell cycle-regulated genes are also under the influence of methylation and GcrA-dependent transcriptional regulation, this could be a mechanism responsible for maintaining the gene transcription dosage during the S-phase.

Keywords: Caulobacter crescentus; CcrM; DNA methylation; GcrA; transcription.

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References

REFERENCES

    1. Ausmees, N., Kuhn, J.R. and Jacobs-Wagner, C. (2003) The bacterial cytoskeleton: an intermediate filament-like function in cell shape. Cell, 115, 705-713.
    1. Biswas, I. and Mohapatra, S.S. (2012) CovR alleviates transcriptional silencing by a nucleoid-associated histone-like protein in Streptococcus mutans. Journal of Bacteriology, 194, 2050-2061. https://doi.org/10.1128/JB.06812-11.
    1. Blossey, R., Cardelli, L. and Phillips, A. (2006) A compositional approach to the stochastic dynamics of gene networks. In: Priami, C., Cardelli, L. and Emmott, S. (Eds.) Transactions on Computational Systems Biology IV. Berlin Heidelberg: Springer, pp. 99-122.
    1. Blossey, R., Cardelli, L. and Phillips, A. (2008) Compositionality, stochasticity, and cooperativity in dynamic models of gene regulation. HFSP Journal, 2, 17-28.
    1. Bowman, G.R., Comolli, L.R., Zhu, J., Eckart, M., Koenig, M., Downing, K.H., et al. (2008) A polymeric protein anchors the chromosomal origin/ParB complex at a bacterial cell pole. Cell, 134, 945-955. https://doi.org/10.1016/j.cell.2008.07.015.

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