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. 2020 Mar 18;13(1):164.
doi: 10.1186/s13104-020-05000-w.

BRAF V600 mutation profiling in primary skin nodular melanoma in Indonesia: an analysis using high resolution pyrosequencing

Hanggoro Tri Rinonce  1 , Rovi Panji Mustiko Aji  2 , Ni'mah Hayati  2 , Maria Fransiska Pudjohartono  2 , Bidari Kameswari  3 , Sumadi Lukman Anwar  4 , Irianiwati  2
Affiliations

BRAF V600 mutation profiling in primary skin nodular melanoma in Indonesia: an analysis using high resolution pyrosequencing

Hanggoro Tri Rinonce et al. BMC Res Notes. .

Abstract

Objective: We aimed to investigate the prevalence and type of BRAF V600 mutations and the associations with clinicopathological variables in primary skin nodular melanoma cases in Yogyakarta and Central Java, Indonesia using pyrosequencing.

Results: BRAF V600 mutations of the V600E type were found in 21 (53.85%) specimens. The variant allele frequencies (VAFs) ranged from 5.07 to 94.70%, averaging 29.05%. However, most cases had low VAFs, with 13 (61.9%) specimens below 20% and 4 (19.05%) below 10%.

Keywords: BRAF; Indonesia; Melanoma; Pyrosequencing; Skin cancer.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
The distribution of BRAF V600 mutation variant allele frequencies
See this image and copyright information in PMC

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