MicroRNAs association with azoospermia, oligospermia, asthenozoospermia, and teratozoospermia: a systematic review

Abstract

Infertility is a major health problem across the world. One of the main reasons for male infertility are defects in sperm. Semen analysis is the most common test utilized to evaluate male fertility and since it suffers from multiple drawbacks, reproduction scientists have tried to find new molecular markers for detecting sperm defects. MicroRNAs (miRNAs) are small molecules in cells which take part in regulating gene expression. Various studies have confirmed miRNAs to have a role in defining multiple sperm characteristics, including sperm count, motility, and morphology. In this paper, we have systematically reviewed the role of miRNAs in infertile men with sperm defects including azoospermia, oligospermia, asthenozoospermia, and teratozoospermia. Also, we have assembled various bioinformatics tools to come up with a pipeline for predicting novel miRNAs which could possibly participate in sperm count, motility, and morphology. Also, related KEGG and GO terms for predicted miRNAs have been included in order to highlight their role in sperm function. Our study emphasizes the potential role of miRNAs in male infertility and provides a general overview for future studies aiming to find robust molecular markers for this condition.

Keywords: Asthenozoospermia; Azoospermia; Bioinformatics; MicroRNA; Oligospermia; Teratozoospermia.

Fig. 1

Pipeline employed in this study

Fig. 2

Study flowchart

Fig. 3

Predicted miRNAs A: AZ, B: OS, C: AZS, D: TS

Fig. 4

Kegg pathways A: AZ, B: OS, C: AZS, D: TS

Fig. 5

GO terms A: AZ, B: OS, C: AZS, D: TS. (blue: biological process, red: molecular function, green: cellular component)