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. 2020 May;62(4):223-231.
doi: 10.1111/dgd.12656. Epub 2020 Mar 18.

Efficiently accumulating germ-like stem cells from mouse postnatal ovary by in situ tissue culture

Meizi Zhang  1 , Li Liu  1 , Xiaomin Cao  1 , Ye Liu  1 , Jianyong Di  1 , Xiuying Huang  2 , Fangzhen Sun  2 , Weihong Huang  2 , Fengqin Xu  1
Affiliations

Efficiently accumulating germ-like stem cells from mouse postnatal ovary by in situ tissue culture

Meizi Zhang et al. Dev Growth Differ. 2020 May.

Abstract

Although recent studies have revealed that germline stem cells (GSCs) exist in the mouse postnatal ovary, how to efficiently obtain GSCs for regenerating neo-oogenesis is still a technical challenge. Here, we report that using in situ tissue culture we can efficiently accumulate large amounts of proliferating germ-like cells from mouse postnatal ovaries. Usually, more than 10,000 germ-like cells can be derived from one ovary by this method, and over 20% of these cells can grow into germ-like cells with self-renewal, which thus can serve as a good cell pool to isolate GSCs by other cell assorting methods such as FACS. This method is simple and time-saving, which should be useful for in future studies on mouse GSCs.

Keywords: germline stem cells; in situ tissue culture; postnatal mouse ovary; proliferation.

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References

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