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. 2020 Mar 3:2020:4736152.
doi: 10.1155/2020/4736152. eCollection 2020.

Construction and Chemical Profile on "Activity Fingerprint" of Citri Reticulatae Pericarpium from Different Cultivars Based on HPLC-UV, LC/MS-IT-TOF, and Principal Component Analysis

Guodong Zheng  1 , Yingxin Chao  1 , Meixia Luo  1   2 , Bin Xie  3 , Dedong Zhang  3 , Pingjun Hu  1 , Xiujuan Yang  1 , Depo Yang  4 , Minyan Wei  1
Affiliations

Construction and Chemical Profile on "Activity Fingerprint" of Citri Reticulatae Pericarpium from Different Cultivars Based on HPLC-UV, LC/MS-IT-TOF, and Principal Component Analysis

Guodong Zheng et al. Evid Based Complement Alternat Med. .

Abstract

Citri Reticulatae Pericarpium (CRP), known as Chenpi (CP) in Chinese, is a medicinal food for health and fitness. In order to find out the characteristic activity chemicals distinguishing various cultivars of CRP and provide a reference for effective development of citrus resources, an "activity fingerprint" of CRP from 21 different cultivars was established based on the evaluation of antitussive and expectorant activities. There were 18 common peaks in the HPLC fingerprint, of which 3 flavonoid glycosides and 14 polymethoxyflavonoids (PMFs) were identified by LC/MS-IT-TOF. Furthermore, five characteristic chemicals were determined and similarity calculation with principal component analysis (PCA) was combined together to compare the similarity and difference among various cultivars. The results showed that some other cultivars were also similar to C. reticulata "Chachi" except for C. reticulata "Tangerina" and C. reticulata "Dahongpao" recorded in Chinese Pharmacopoeia. Most importantly, the peels of C. reticulata "Shiyueju," C. reticulata "Ponkan," C. reticulata "Tribute," and C. reticulata "Bayueju," traditionally rarely used for medicinal food, were highly similar to that of C. reticulata "Chachi" and rich in bioactive flavonoids, which can be considered the effective medicinal resources of CRP.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
Effect of the MECRP on ammonia water-irritated cough and tracheal phenol red excretion in mice. (a) Incubation period of cough. (b) Bouts of cough. (c) Tracheal phenol red excretion. All values are expressed as mean ± SD (n = 10). ∗P < 0.05 and ∗∗P < 0.01 compared to the control group (one-way ANOVA followed by the LSD test).
Figure 2
Figure 2
(a) GCP fingerprint of samples S1–S12. (b) GCP common pattern R. (c) Fingerprint of representative cultivars of CRP. Peaks 3, 9, and 12 are hesperidin, nobiletin, and tangeretin, respectively.
Figure 3
Figure 3
(a) Total ion chromatogram (TIC) of CRP in the positive mode. (b) TIC of mixed standard solution in the positive mode. Peaks 3, 9, 11, 12, and 14 are hesperidin, nobiletin, 3,5,6,7,8,3′,4′-heptamethoxyflavone, tangeretin, and 5-hydroxy-6,7,8,3′,4′-pentamethoxyflavone, respectively.
Figure 4
Figure 4
PCA results of CRP from different cultivars.
See this image and copyright information in PMC

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