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. 2020 Feb 16:34:100657.
doi: 10.1016/j.nmni.2020.100657. eCollection 2020 Mar.

Dialister massiliensis sp. nov., a new bacterium isolated from the human gut

P Afouda  1   2 , G Dubourg  1   2 , E Tomeï  3   2 , D Raoult  1   2   4 , P-E Fournier  3   2
Affiliations

Dialister massiliensis sp. nov., a new bacterium isolated from the human gut

P Afouda et al. New Microbes New Infect. .

Abstract

Dialister massiliensis strain Marseille-P5638T (= CSUR P5638) is a new species from the genus Dialister and family Veillonellaceae which was isolated from the gut microbiota of a healthy individual.

Keywords: Culturomics; Dialister massiliensis sp. nov.; Firmicutes; gut microbiota; taxonogenomics.

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Figures

Fig. 1
Fig. 1
MALDI-TOF MS reference spectrum of Dialister massiliensis sp. nov. strain Marseille-P5638T. Reference spectrum was generated by comparison of spectra from 12 individual colonies.
Fig. 2
Fig. 2
Transmission electron micrograph of Dialister massiliensis sp. nov. strain Marseille-P5638T (a) Bacterial cells in pairs. (b) Bacterial cells in quadruplet. Colony was collected from agar and fixed with 2.5% glutaraldehyde in 0.1 M cacodylate buffer for at least 1 hour at 4°C. Drop of cell suspension was deposited for approximately 5 minutes on glow-discharged formvar carbon film with 400 mesh nickel grids (FCF400–Ni, EMS). Grids were dried on blotting paper and cells were negatively stained for 10 seconds with 1% ammonium molybdate solution in filtered water at room temperature. Electron micrographs were acquired with a Tecnai G20 Cryo (FEI Company, Limeil-Brévannes, France) transmission electron microscope operated at 200 keV. Scale bar represents 500 nm.
Fig. 3
Fig. 3
Phylogenetic tree showing position of Dialister massiliensis sp. nov., strain Marseille-P5638T, relative to other phylogenetically close neighbours. GenBank accession numbers of 16S ribosomal RNA are indicated in parentheses. Sequences were aligned using MUSCLE with default parameters; phylogenetic inferences were obtained by maximum composite likelihood method and MEGA 6 software. Bootstrap values obtained by repeating analysis 1000 times to generate majority consensus tree are indicated at nodes. Only bootstrap values ≥ 90% were retained. Scale bar indicates 0.5% nucleotide sequence divergence.
Fig. 4
Fig. 4
Heat map generated by OrthoANI values calculated by OAT software between Dialister massiliensis sp. nov. strain Marseille-P5638T and other closely related species with standing in nomenclature.
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