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Review
. 2020 Sep;97(9):891-899.
doi: 10.1002/cyto.a.24001. Epub 2020 Mar 21.

Comprehensive Phenotyping of Human PB NK Cells by Flow Cytometry

Genny Del Zotto  1 , Francesca Antonini  1 , Silvia Pesce  2 , Francesca Moretta  3 , Lorenzo Moretta  4 , Emanuela Marcenaro  2   5
Affiliations
Free article
Review

Comprehensive Phenotyping of Human PB NK Cells by Flow Cytometry

Genny Del Zotto et al. Cytometry A. 2020 Sep.
Free article

Abstract

The NK cell compartment provides powerful innate defenses against virus-infected and tumor cells. Specific NK cell receptors control this process and maintain the immune system homeostasis and prevent autoimmunity. A wide variety of NK cell subsets with different functional capabilities exist and this reflects not only the different maturation stages of NK cells but also different microenvironments in which they can operate. In this review, we will give an overview on the various NK cell subsets present in peripheral blood of healthy donors in order to clearly and univocally identify them on the basis of their phenotypic traits using flow cytometry. © 2020 International Society for Advancement of Cytometry.

Keywords: NK cell subsets; flow cytometry; human NK cells; phenotyping.

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