Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2020 May;30(5):372-373.
doi: 10.1038/s41422-020-0297-6.

A separation that's for the best: coming together at the PAS

Wayne D Hawkins  1   2 , Daniel J Klionsky  3   4
Affiliations
Comment

A separation that's for the best: coming together at the PAS

Wayne D Hawkins et al. Cell Res. 2020 May.
No abstract available

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1. Diagram of the phase separation of the PAS components and the regulation of this phenomenon by cellular cues.
Under high nutrient availability, TORC1 is active and phosphorylates several autophagy proteins including Atg13 at residues S428 and S429, modifications that impair the Atg13-Atg17 interaction, precluding PAS formation in growing conditions. When cells are starved for nutrients, TORC1 is inactivated and the inhibitory phosphoryl groups placed on Atg13 by this complex are removed by PP2C phosphatases. This dephosphorylation allows formation of the PAS droplet resulting in the clustering of Atg1 molecules, which contributes to activating autophosphorylation of this kinase. Activated Atg1 then re-phosphorylates S428 and S429 on Atg13; however, this is reversed by PP2C phosphatases such that the PAS condensate remains stable. The peri-vacuolar localization of the PAS is facilitated at least partially by the interactions between Atg13 and Vac8.
See this image and copyright information in PMC

Comment on

References

    1. Kim J, Huang WP, Stromhaug PE, Klionsky DJ. J. Biol. Chem. 2002;277:763–773. doi: 10.1074/jbc.M109134200. - DOI - PMC - PubMed
    1. Suzuki K, et al. EMBO J. 2001;20:5971–5981. doi: 10.1093/emboj/20.21.5971. - DOI - PMC - PubMed
    1. Kawamata T, Kamada Y, Kabeya Y, Sekito T, Ohsumi Y. Mol. Biol. Cell. 2008;19:2039–2050. doi: 10.1091/mbc.e07-10-1048. - DOI - PMC - PubMed
    1. Cheong H, Nair U, Geng J, Klionsky DJ. Mol. Biol. Cell. 2008;19:668–681. doi: 10.1091/mbc.e07-08-0826. - DOI - PMC - PubMed
    1. Fujioka Y, et al. Nat. Struct. Mol. Biol. 2014;21:513–521. doi: 10.1038/nsmb.2822. - DOI - PubMed

MeSH terms