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Published Erratum
. 2020 Mar 31;117(13):7537-7538.
doi: 10.1073/pnas.2003767117. Epub 2020 Mar 23.

Correction for Krieger et al., Mutations in thyroid hormone receptor α1 cause premature neurogenesis and progenitor cell depletion in human cortical development

No authors listed
Published Erratum

Correction for Krieger et al., Mutations in thyroid hormone receptor α1 cause premature neurogenesis and progenitor cell depletion in human cortical development

No authors listed. Proc Natl Acad Sci U S A. .
No abstract available

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Figures

Fig. 4.
Fig. 4.
FACS-enriched THRA mutation-containing cortical progenitor cells generate functional neuronal networks. (A) Representative FACS results for WT and THRA mutation-containing cells at day 18. Immunocytochemistry confirmed the cortical identity of FACS- selected progenitor cells. (Scale bars: 50 μm.) (B) Neurons produced by FACS-purified progenitor cells expressed the cortical layer-specific transcription factors TBR1 (layer VI) and CTIP2 (layer V), as well as the vesicular glutamate transporter vGlut1, at day 40. (Scale bars: 50 μm.) (C) Representative traces showing action potential firing in response to stepwise current injection in THRA mutation-containing and control neurons. Numbers indicate the proportion of cells that fired action potentials. (D) Calcium indicator Oregon Green BAPTA was used as a proxy for action potential firing to measure spontaneous neuronal activity. Fluorescence images show control and THRA mutant cultures after loading. Heat maps highlight different levels of activity across the cultures. Total calcium activity across the field of view, ACa, was quantified based on 3–6 videos from each cell line (403X and FS382, n = 6 samples from 2 independent inductions; all others, n = 3 samples from 1 induction; *P < 0.05, Student’s t test). Error bars indicate SEM.
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