Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020:2140:159-170.
doi: 10.1007/978-1-0716-0520-2_10.

3D Bioprinting Electrically Conductive Bioink with Human Neural Stem Cells for Human Neural Tissues

Eva Tomaskovic-Crook  1   2 , Jeremy M Crook  3   4   5
Affiliations

3D Bioprinting Electrically Conductive Bioink with Human Neural Stem Cells for Human Neural Tissues

Eva Tomaskovic-Crook et al. Methods Mol Biol. 2020.

Abstract

Bioprinting cells with an electrically conductive bioink provides an opportunity to produce three-dimensional (3D) cell-laden constructs with the option of electrically stimulating cells in situ during and after tissue development. We and others have demonstrated the use of electrical stimulation (ES) to influence cell behavior and function for a more biomimetic approach to tissue engineering. Here, we detail a previously published method for 3D printing an electrically conductive bioink with human neural stem cells (hNSCs) that are subsequently differentiated. The differentiated tissue constructs comprise functional neurons and supporting neuroglia and are amenable to ES for the purposeful modulation of neural activity. Importantly, the method could be adapted to fabricate and stimulate neural and nonneural tissues from other cell types, with the potential to be applied for both research- and clinical-product development.

Keywords: 3D bioprinting; Electrical stimulation; Electrically conductive bioink; Human neural tissue; Stem cells.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Crook JM, Wallace G, Tomaskovic-Crook E (2015) The potential of induced pluripotent stem cells in models of neurological disorders: implications on future therapy. Expert Rev Neurother 15(3):295–304 - DOI - PubMed
    1. Dean B (2009) Is schizophrenia the price of human central nervous system complexity? Aust N Z J Psychiatry 43:13–24 - DOI - PubMed
    1. Gu Q, Tomaskovic-Crook E, Lozano R, Chen Y, Kapsa RM, Zhou Q et al (2016) Functional 3D neural mini-tissues from printed gel-based human neural stem cells. Adv Healthc Mater 5:1429–1438 - DOI - PubMed
    1. Tomaskovic-Crook E, Zhang P, Ahtiainen A, Kaisvuo H, Lee CY, Beirne S et al (2019) Human neural tissues from neural stem cells using conductive biogel and printed polymer microelectrode arrays for 3D electrical stimulation. Adv Healthc Mater 8(15):1900425 - DOI
    1. Crook JM, Tomaskovic-Crook E (2017) Culturing and cryobanking human neural stem cells. In: Crook JM, Ludwig TE (eds) Stem cell banking: concepts and protocols, Methods in molecular biology, vol 1590. Human Press, New York - DOI

Publication types

LinkOut - more resources