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. 2020:2140:251-258.
doi: 10.1007/978-1-0716-0520-2_17.

Bioprinting 3D Human Induced Pluripotent Stem Cell Constructs for Multilineage Tissue Engineering and Modeling

Jeremy M Crook  1   2   3 , Eva Tomaskovic-Crook  4   5
Affiliations

Bioprinting 3D Human Induced Pluripotent Stem Cell Constructs for Multilineage Tissue Engineering and Modeling

Jeremy M Crook et al. Methods Mol Biol. 2020.

Abstract

Bioprinting human pluripotent stem cells (PSCs) provides an opportunity to produce three-dimensional (3D) cell-laden constructs with the potential to be differentiated in vitro to all tissue types of the human body. Here, we detail a previously published method for 3D printing human induced pluripotent stem cells (iPSCs; also applicable to human embryonic stem cells) within a clinically amenable bioink (also described in Chapter 10 ) that is cross-linked to a 3D construct. The printed iPSCs continue to have self-replicating and multilineage cell induction potential in situ, and the constructs are robust and amenable to different differentiation protocols for fabricating diverse tissue types, with the potential to be applied for both research- and clinical-product development.

Keywords: 3D bioprinting; Clinically amenable bioink; Human induced pluripotent stem cells; Multilineage; Tissue.

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References

    1. Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS et al (1998) Embryonic stem cell lines derived from human blastocysts. Science 282(5391):1145–1147 - DOI - PubMed
    1. Crook JM, Peura T, Kravets L, Bosman A, Buzzard JJ, Horne R et al (2007) The generation of six clinical-grade human embryonic stem cell lines. Cell Stem Cell 1:490–494 - DOI - PubMed
    1. Takahashi K, Tanabe K, Ohnuki M, Ichisaka T, Tomoda K, Yamanaka S (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131(5):861–872 - DOI - PubMed
    1. Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S et al (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318(5858):1917–1920 - DOI - PubMed
    1. Gu Q, Tomaskovic-Crook E, Wallace GG, Crook JM (2017) 3D bioprinting human induced pluripotent stem cell constructs for in situ cell proliferation and successive multi-lineage differentiation. Adv Healthc Mater 6:1700175 - DOI

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