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. 2020 Feb 20:7:100814.
doi: 10.1016/j.mex.2020.100814. eCollection 2020.

A minimally invasive technique for serial intraosseous perfusion measurements in the murine tibia using laser Doppler flowmetry

Nicholas J Hanne  1 , Elizabeth D Easter  2 , Sandra Stangeland-Molo  1 , Jacqueline H Cole  1
Affiliations

A minimally invasive technique for serial intraosseous perfusion measurements in the murine tibia using laser Doppler flowmetry

Nicholas J Hanne et al. MethodsX. .

Abstract

In biomedical and preclinical research, the current standard method for measuring blood perfusion inside murine bone, radiolabeled microspheres, is a terminal procedure that cannot be used to monitor longitudinal perfusion changes. Laser Doppler flowmetry (LDF) can assess perfusion within the proximal tibial metaphysis of mice in vivo but requires a surgical procedure to place the measurement probe directly onto the bone surface. Sustained inflammation for over a month following this technique was previously reported, and previous studies have used LDF as an endpoint-only procedure. We developed a modified, minimally invasive LDF procedure to measure intraosseous perfusion in the murine tibia without stimulating local or systemic inflammation or inducing gait abnormalities. This modified technique can be used to measure perfusion weekly for up to at least a month in the murine tibia.•Unlike previous endpoint-only techniques, this modified LDF procedure can be performed weekly to monitor serial changes to intraosseous perfusion in the murine tibia•The modified LDF technique utilizes a smaller, more localized incision to minimize invasiveness and speed recovery.

Keywords: Bone blood flow; In vivo; Vascular supply.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Image, graphical abstract
Graphical abstract
Fig. 1:
Fig. 1
Image showing the measurement site on the right hindlimb (A) before and (B) after the incision is made. The arrows point to the approximate position of the right knee.
Fig. 2:
Fig. 2
Image showing probe placement during the modified laser Doppler flowmetry technique on the right hindlimb. The arrow points to the approximate position of the right knee.
Fig. 3:
Fig. 3
Perfusion measured in the proximal tibia was similar between mice that received a weekly laser Doppler flowmetry procedure for four weeks (Repeated) and mice that received the procedure only once at the final timepoint (Endpoint).
See this image and copyright information in PMC

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