Molecular Prevalence and Phylogenetic Characterization of Enterocytozoon bieneusi in Healthy Cattle

Abstract

Objective: In this study, it was aimed to determine the molecular prevalence and genotypes of Enterocytozoon in healthy cattle.

Methods: Fecal samples were collected from 50 cattle in Sivas between October 2017 and March 2018 and genomic DNA (gDNA) isolations were performed. gDNA isolates were processed by Nested PCR specifically amplifying ITS rRNA gene region to identify E. bieneusi. ITS rRNA region of E. bieneusi positive isolates were sequenced for genotyping and phylogenetic analyzes. Obtained sequences were assembled with appropriative genetic software, then phylogenetic relationships were revealed.

Results: According to Nested PCR analyses, 29 (19.3%) out of totally examined samples were found positive for E. bieneusi. As a result of the sequence analyses, five distinct genotypes were determined. The most frequent genotype ERUSS1 and the other ERUSS2-4 genotypes were characterized as close to each other, which was reported for the first time in the world. Two isolates were determined in N genotype that was reported from cattle in Germany and were more different from the other genotypes. Phylogenetic analysis revealed that all the genotypes characterized in the study belonged to the genogroup 2.

Conclusion: First molecular epidemiological data on E. bieneusi in cattle from Turkey were obtained with this study.

Keywords: Enterocytozoon bieneusi; molecular prevalence; phylogenetic characterization; healthy cattle; Turkey.