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. 2010;26(1):75-82.
doi: 10.1007/s10453-009-9144-4. Epub 2009 Nov 26.

Effects of different sampling solutions on the survival of bacteriophages in bubbling aeration

Long Yu  1 , Zhanbo Wen  1 , Jinsong Li  2 , Wenhui Yang  1 , Jie Wang  1 , Na Li  1 , Jianchun Lu  1
Affiliations

Effects of different sampling solutions on the survival of bacteriophages in bubbling aeration

Long Yu et al. Aerobiologia (Bologna). 2010.

Abstract

The present study focuses on the effects of three different sampling solutions, namely distilled water, phosphatic buffer solution, and suspension medium (SM), adding antifoam or not, on the survival probability of several different bacteriophages (EcP1,PhiX174,SM702, and F2) as surrogates for the mammalian viruses in the bubbling process. AGI-10 impinger was used as the representative for all the impingers which would bubble during operation. It was found that the survival probability of the same bacteriophage bubbling with different sampling solutions was different except that there was no significant difference observed for the bacteriophage F2. The use of SM as the collection fluid was relative to a high survival probability for the four bacteriophages. And the endurance or resistance of different kinds of bacteriophages in the same sampling solution was different. We conclude that SM is a promising sampling solution for liquid impingers in the process of sampling phages.

Keywords: Bacteriophage; Bubbling aeration; Impinger; Sampling solutions; Survival percentage correction; Viral surrogate.

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Figures

Fig. 1
Fig. 1
Dynamics of the survival percentage correction during bubbling aeration with AGI-10 impinger. Each point represents the mean percentage value, and the error bars represent the standard deviation
See this image and copyright information in PMC

References

    1. Ackermann, H. W. (1999). Bacteriophages. In J. P. Lederberg (Ed.), Encylopedia of microbiology (2nd ed., Vol. 1, pp. 398–411). New York: Academic Press.
    1. Ackermann HW. Bacteriophage classification. In: Kutter E, Sulakvelidze A, editors. Bacteriophages: Biology and applications. Boca Raton: CRC Press; 2005. pp. 67–89.
    1. Adams MH. Surface inactivation of bacterial viruses and of proteins. Journal of General Physiology. 1948;31:417–431. doi: 10.1085/jgp.31.5.417. - DOI - PMC - PubMed
    1. Adams MA. Bacteriophages. New York: Interscience Publishers, Inc; 1959.
    1. Agranovski IE, Safatov AS, Borodulin AI, Pyankov OV, Petrishchenko VA, Sergeev AN, Agafonov AP, Ignatiev GM, Sergeev AA, Agranovski V. Inactivation of viruses in bubbling processes utilized for personal bioaerosol monitoring. Applied and Environmental Microbiology. 2004;70:6963–6967. doi: 10.1128/AEM.70.12.6963-6967.2004. - DOI - PMC - PubMed

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