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. 2012;21(4):475-478.
doi: 10.1007/s00580-010-1122-2. Epub 2010 Nov 3.

Detection of enterotoxigenic K99 (F5) and F41 from fecal sample of calves by molecular and serological methods

Zahra Shams  1 , Yahya Tahamtan  2 , Ali Pourbakhsh  2 , Mohammad H Hosseiny  2 , Mohammad Kargar  1 , Masoumeh Hayati  2
Affiliations

Detection of enterotoxigenic K99 (F5) and F41 from fecal sample of calves by molecular and serological methods

Zahra Shams et al. Comp Clin Path. 2012.

Abstract

Enterotoxigenic Escherichia coli (ETEC) is one of the major causes of neonatal calf diarrhea. Almost all ETEC bacteria are known to adhere to receptors on the small intestinal epithelium via their fimbriae, (F5 (K99) and F41).This study was undertaken to investigate the phenotypic and genotypic screening of virulence genes in E. coli K99 and F41. During January 2008 to December 2009, 298 diarrheic neonatal calves at 1-30 days old were studied by multiplex PCR, isolation, and serological grouping. Of the 298 diarrheic samples, 268 E. coli were isolated, of which 16 samples (5.3%) were positive for having the F5 (K99) fimbrial gene by PCR while all of the E. coli isolates also carried F41 fimbrial genes. Twenty-five percent of the isolates were proven not to be toxigenic as they did not possess the STa enterotoxin gene.

Keywords: Cattle; E. coli K99; F41; F5; Iran.

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References

    1. Acha SJ, Kuhn I, Jonsson P, Mbazima G, Katouli M. Studies on calf diarrhea in Mozambique: prevalence of bacterial pathogens. Acta Vet Scand. 2004;45:27–36. doi: 10.1186/1751-0147-45-27. - DOI - PMC - PubMed
    1. Akam A, Khelef D, Kaidi R, Othmani A, Lafri M, Tali-Maamar H, Rahal K, Tahrat N, Chirila F, Cozma V, Abdul-Hussain MS. Frequency of Cryptosporidium parvum, Escherichia coli K99 and Salmonella spp.isolated from healthy and unhealthy calves in six breeding farms from Mitidja, Algeria (preliminary results) Rev Sci Parasitol. 2004;5:13–21.
    1. Barrington GM, Gay JM, Evermann JF. Biosecurity for neonatal gastrointestinal diseases. The veterinary clinics of North America. Food Anim Pract. 2002;18:7–34. doi: 10.1016/S0749-0720(02)00005-1. - DOI - PMC - PubMed
    1. Crouch CF, Oliver S, Francis MJ. Serological, colostral and milk responses of cows vaccinated with a single dose of a combined vaccine against rotavirus, coronavirus and Escherichia coli F5 (K99) Vet Rec. 2001;12:149–153. - PubMed
    1. Guinee PAM, Jansen WH, Agterberg CM. Detection of the K99 antigen by means of agglutination and immunoelectrophoresis in Escherichia coli isolates from calves and its correlation with enterotoxigenicity. Infect Immun. 1976;13:1369–1377. - PMC - PubMed

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