Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007;3(1):23-30.
doi: 10.1007/s12030-007-0003-5. Epub 2007 May 3.

Detection of human viral RNA via a combined fluorescence and SERS molecular beacon assay

M Y Sha  1 , S Penn  1 , G Freeman  1 , W E Doering  1
Affiliations

Detection of human viral RNA via a combined fluorescence and SERS molecular beacon assay

M Y Sha et al. Nanobiotechnology. 2007.

Abstract

A dual-mode molecular beacon on a multiplexed substrate has been developed and applied to the measurement of unlabeled human viral RNA. The detection system is based on a combined surface-enhanced Raman scattering (SERS) and fluorescent molecular beacon assay that is assembled on Nanobarcodes™ particles. In this assay, a molecular beacon probe terminated with a fluorescent Raman label dye is conjugated to the metallic Nanobarcodes™ particles. The molecular beacon probe is a single-stranded oligonucleotide that has been designed with a hairpin structure that holds the dye at 3'-end close to the particle surface when the probe is attached through a 5'-thiol group. In this configuration, the SERS spectrum of the label was obtained and its fluorescence quenched because the dye is in very close proximity to a noble metal surface with nanoscale features (Nanobarcodes™ particles). The SERS signal decreased and the fluorescence signal increased when target viral RNA was captured by this molecular beacon probe. In addition, a hepatitis C virus reverse transcriptase-polymerase chain reaction (HCV RT-PCR) product was detected using this dual-mode beacon. The development of a multiplexed, label-free assay system with the reassurance offered by detection of two distinctly separate signals offers significant benefits for rapid molecular diagnostics.

Keywords: RNA detection; SERS beacon; label-free; molecular beacon.

PubMed Disclaimer

References

    1. Fleischman M, Hendra PJ, McQuillan AJ. Chem Phys Lett. 1974;26:163–6. doi: 10.1016/0009-2614(74)85388-1. - DOI
    1. Jeanmaire DL, Van Duyne RP. J Electroanal Chem. 1977;84:1–20. doi: 10.1016/S0022-0728(77)80224-6. - DOI
    1. Cotton TM, Schulz SG, Van Duyne RP. J Am Chem Soc. 1980;102:7960–2. doi: 10.1021/ja00547a036. - DOI
    1. Zhange X, Young MA, Lyandres O, Van Duyne RP. J Am Chem Soc. 2005;127:4484–9. doi: 10.1021/ja043623b. - DOI - PubMed
    1. Culha M, Stokes D, Allain L, Vo-Dinh T. Anal Chem. 2003;75:6196–201. doi: 10.1021/ac0346003. - DOI - PubMed

LinkOut - more resources