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. 2019 Nov 24;7(1):51-55.
doi: 10.5455/javar.2020.g392. eCollection 2020 Mar.

Polymorphism of CLPG gene in three sheep breeds grown in the steppe zone of the Russian Federation

Ivan Fiodorovich Gorlov  1   2 , Nadezhda Vasilievna Shirokova  1 , Yuri Anatolievich Kolosov  3 , Anatoli Yurievich Kolosov  3 , Lyubov Vladimirovna Getmantseva  4 , Marina Ivanovna Slozhenkina  1   2 , Natalia Ivanovna Mosolova  1   2 , Elena Yurievna Anisimova  1   5 , Viktor Vladimirovich Ponomariov  1
Affiliations

Polymorphism of CLPG gene in three sheep breeds grown in the steppe zone of the Russian Federation

Ivan Fiodorovich Gorlov et al. J Adv Vet Anim Res. .

Abstract

Objective: This study aims to investigate the callipyge gene (CLPG) polymorphism in sheep of Edilbay, Volgograd, and Kalmyk breeds.

Materials and methods: The analysis was performed by the polymerase chain reaction-restriction fragment length polymorphisms method. The objects of the study were Edilbay fat-tailed sheep (n = 500) at the breeding plant Volgograd-Edilbay (Volgograd region), Volgograd fine-wool sheep (n = 500) at the breeding plant Romashkovskiy (Volgograd region), and Kalmyk fat-tailed sheep (n = 500) at the breeding plant Kirovsky (the Republic of Kalmykia, Yashkul rayon). To conduct the research, tissue samples of 1 cm² from sheep of Kalmyk and Edilbay breeds were taken from the auricle.

Results: The allelic CLPG gene variants have been determined and genotypes of representative sampling of the three breeds of livestock grown in the steppe zone of Russia. The presented results of the CLPG gene polymorphism in these sheep breeds grown in Russia were obtained for the first time. The research study has revealed that in terms of the CLPG gene, the Edilbay, Volgograd, and Kalmyk sheep breeds have only a homozygous form.

Conclusion: The results obtained expand the current understanding of the molecular markers that characterize the meat qualities of sheep.

Keywords: Callipyge; PCR-RFLP; Sheep Breeding; marker-assisted selection; single-nucleotide polymorphism.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Figure 1.
Figure 1.. Electropherograms of the PCR-RFLP result of the CLPG/BsmF1 gene in 2% agarose gel. (A) Edilbay fat-tailed sheep breed; (B) Volgograd fine-wool sheep breed; (C) Kalmyk fat-tailed sheep breed; M is DNA marker (50+ bp DNA Ladder, Evrogen, Russia).
See this image and copyright information in PMC

References

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