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. 2011;52(5):511.
doi: 10.1007/s13580-011-0007-x. Epub 2011 Oct 28.

Expression of the protective antigen for PEDV in transgenic duckweed, Lemna minor

Suk-Min Ko  1 , Hyeon-Jin Sun  1 , Myung Jin Oh  2 , In-Ja Song  1 , Min-Jae Kim  1 , Hyun-Sook Sin  1 , Chang-Hyo Goh  3 , Yong-Woo Kim  1 , Pyung-Ok Lim  1   4 , Hyo-Yeon Lee  1   5 , Suk Weon Kim  2
Affiliations

Expression of the protective antigen for PEDV in transgenic duckweed, Lemna minor

Suk-Min Ko et al. Hortic Environ Biotechnol. 2011.

Abstract

Duckweeds are small, floating aquatic plants with a number of useful characteristics, including edibility, fast-growing, and a clonal proliferation. Duckweed is also fed to animals as a diet complement because of its high nutritional value. Porcine epidemic diarrhea virus (PEDV) is a major causative agent of fatal diarrhea in piglets and is a serious problem in the hog-raising industry. In this study, we assessed the feasibility of producing a protective antigen for the PEDV spike protein 1 using duckweed, Lemna minor. Stably transformed Lemna were obtained by co-cultivation with A. tumefaciens EHA105 harboring the PEDV spike protein gene. Transgene integration and expression of the PEDV spike protein 1 gene were confirmed by genomic PCR and RT-PCR and western blot analysis of transgenic Lemna, respectively. This is the first report of the expression of a vaccine antigen against an animal infectious disease in duckweed.

Keywords: aquatic plant; porcine diarrhea; spike protein; transformation; vaccine.

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References

    1. Chauvin J.E., Marhadour S., Cohat J., Le Nard M. Effects of gelling agent on in vitro regeneration and kanamycin efficiency as a selective agent in plant transformation procedures. Plant Cell Tiss. Organ Cult. 1999;58:213–217. doi: 10.1023/A:1006390929364. - DOI
    1. Coussement W., Ducatelle R., Debouck P., Hoorens J. Pathology of experimental CV777 coronavirus enteritis in piglets. Vet Pathol. 1982;19:46–56. doi: 10.1177/030098588201900108. - DOI - PubMed
    1. Cox K.M., Sterling J.D., Regan J.T., Gasdaska J.R., Frantz K.K., Peele C.G., Black A., Passmore D., Moldovan-Loomis C., asan M. S., Cuison S., Cardarelli P.M., Dicky L.F. Glycan optimization of a human monoclonal antibody in the aquatic plant Lemna minor. Nature Biotechnol. 2006;24:1591–1597. doi: 10.1038/nbt1260. - DOI - PubMed
    1. Fischer, R., E. Stoger, S. Schillberg, P. Christou, and R.M. Twyman. 004. Plant-based production of biopharmaceuticals. Curr. Opin. lant Biol. 7:152–158. - PubMed
    1. Gasdaska, J.R., D. Spencer, and L. Dickey. 2003. Advantage of therap eutic protein production in aquatic plant Lemna. BioProcess. J.:50–56.

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