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. 2020 Mar 28;25(7):1555.
doi: 10.3390/molecules25071555.

Anthocyanin Induction by Drought Stress in the Calyx of Roselle Cultivars

Jeny Hinojosa-Gómez  1 , César San Martín-Hernández  2 , José B Heredia  1 , Josefina León-Félix  1 , Tomás Osuna-Enciso  1 , María D Muy-Rangel  1
Affiliations

Anthocyanin Induction by Drought Stress in the Calyx of Roselle Cultivars

Jeny Hinojosa-Gómez et al. Molecules. .

Abstract

Abiotic factors can alter the chemical profile of crops and the number of compounds they contain. In this study, the anthocyanin and anthocyanidin contents, determined by ultra-performance liquid chromatography (UPLC-MS/MS), and the colour attributes of the calyces of three cultivars of Hibiscus sabdariffa subjected to three water stress regimes during the stage of physiological maturity were investigated. The total anthocyanin content in calyx increased relative to the control content under a 65% moisture irrigation regime. Among the cultivars, UAN16-2 showed the greatest increases in the contents of cyanidin, delphinidin 3-O-glucoside, cyanidin 3-O-glucoside, and cyanidin 3-O-sambubioside. The content of cyanidin 3-O-sambubioside showed the greatest increase, increasing by 55% relative to the control level. The contents of these compounds are correlated with colour attributes such as luminosity. Water stress under the 33% moisture condition during plant development led to decreased anthocyanin contents in all of the roselle cultivars.

Keywords: Hibiscus sabdariffa; UPLC-MS/MS analysis; anthocyanins; colour; drought stress; greenhouse.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Chromatograms of anthocyanins and anthocyanidins determined in roselle calyx extracts.
Figure 2
Figure 2
Mass spectra with a collision energy of 10 V for the identification of anthocyanins and anthocyanidins.
Figure 3
Figure 3
Color parameters and calyx appearance of different roselle genotypes in three irrigation regimes (IR1 = 100%, IR2 = 65%, and IR3 = 33% humidity). Means with different letters within columns and rows per response variable show significant differences according to the Tukey test (p ≤ 0.05).
Figure 4
Figure 4
Weekly average of relative humidity (RH) and maximum (MAXT) and minimum temperatures (MINT) during the cultivation of roselle.
Figure 5
Figure 5
Water retention curve of the substrate.
See this image and copyright information in PMC

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