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. 2020 Mar 12:2020:4275194.
doi: 10.1155/2020/4275194. eCollection 2020.

Reactive Carbonyls Induce TOR- and Carbohydrate-Dependent Hormetic Response in Yeast

Halyna Semchyshyn  1
Affiliations

Reactive Carbonyls Induce TOR- and Carbohydrate-Dependent Hormetic Response in Yeast

Halyna Semchyshyn. ScientificWorldJournal. .

Abstract

The induction of the beneficial and detrimental effects by reactive carbonyl species in yeast has been investigated. In this study, we have presented evidence that glyoxal and methylglyoxal at low concentrations were able to induce a hormetic adaptive response in glucose-grown but not fructose-grown yeast. The hormetic effect was also TOR-dependent. The mutation in genes encoding either TOR1 or TOR2 protein makes yeast highly sensitive to both α-dicarbonyls studied. Simultaneous disruption of TOR1 and TOR2 resulted in higher yeast sensitivity to the α-dicarbonyls as compared to parental cells, but double mutant survived better under carbonyl stress than its single mutant counterparts. The data obtained are consistent with the previous works which reported high toxicity of the α-dicarbonyls and extend them with the report on the beneficial TOR-dependent hormetic effect of glyoxal and methylglyoxal.

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Conflict of interest statement

The author declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Figures

Figure 1
Figure 1
Chemical structures of α-dicarbonyl compounds: glyoxal and methylglyoxal.
Figure 2
Figure 2
Effect of glyoxal on the reproductive ability of S. cerevisiae JK9-3da wild type (a) and its mutants TOR1 (b), TOR 2 (c), and TOR1 TOR2 (d). Results are shown as the mean ± SEM (n = 3–6).
Figure 3
Figure 3
Effect of methylglyoxal on the reproductive ability of S. cerevisiae JK9-3da wild type (a) and its mutants TOR1 (b), TOR2 (c), and TOR1 TOR2 (d). Results are shown as the mean ± SEM (n = 3–6).
See this image and copyright information in PMC

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