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. 2020 Jun;9(11):3974-3982.
doi: 10.1002/cam4.3019. Epub 2020 Mar 30.

Proteomic and genomic biomarkers for Non-Small Cell Lung Cancer: Peroxiredoxin, Haptoglobin, and Alpha-1 antitrypsin

Zahra Najafi  1 , Abdolreza Mohamadnia  2   3 , Rahim Ahmadi  1 , Minoo Mahmoudi  1 , Naghmeh Bahrami  4   5 , Adnan Khosravi  6 , Hamidreza Jamaati  2 , Payam Tabarsi  7 , Mehdi Kazem Pour Dizaji  8 , Sadegh Shirian  9   10   11
Affiliations

Proteomic and genomic biomarkers for Non-Small Cell Lung Cancer: Peroxiredoxin, Haptoglobin, and Alpha-1 antitrypsin

Zahra Najafi et al. Cancer Med. 2020 Jun.

Abstract

Background: The development of lung cancer is a multifactorial process that involves the environmental and genetic factors. The mortality rate of this cancer is higher than breast, colorectal, and prostate cancers. In this study, we try to analyze the proteome of patients with Non-Small Cell Lung Cancer (NSCLC) and compare it with the healthy samples.

Methods: This study has compared 30 lung tissue samples from patients with NSCLC and 30 healthy samples using proteomics and RT-PCR. Hence, tissue samples were obtained from the surgical ward in sterile conditions, and then, protein extraction applied to them. At the next stage, two-dimensional electrophoresis and mass spectrometry LCMS/MS were performed for protein isolation and sequencing, respectively.

Results: The proteome analysis identified more than 40 differences in proteomic pattern of normal lung tissues compared to lung tissues with NSCLC. Peroxiredoxin, Haptoglobin, and Alpha-1 antitrypsin proteins were identified. Molecularly, it has also been shown that the two main proteins of Peroxiredoxin-2 and Alpha-1 antitrypsin were upregulated, and the expression of Haptoglobin protein was downregulated in cancer tissue.

Conclusion: The results of this study showed that there are some differences in term of protein content between the normal and cancerous lung tissues. Further studies are needed to evaluate these proteins that investigate whether these proteins can candidate as biomarkers to use in the early diagnosis of patients with NSCLC.

Keywords: Alpha-1 antitrypsin; Antioxidant; Haptoglobin; NSCLC; Peroxiredoxin-2; lung cancer; proteomics; tumor markers.

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Conflict of interest statement

It is not declared by the authors.

Figures

FIGURE 1
FIGURE 1
The pattern of polypeptide spot in 70% (or more) of the second‐dimension gel of the patients’ cancerous tissue showed increasing or decreasing expression compared to normal tissue is observed. Arrows and numbers indicate downregulated proteins compared to their matched tumor tissue. Qualified changes is determined by A, B, and C. A molecule has upregulated, whereas B and C molecules have downregulated
FIGURE 2
FIGURE 2
The positive percentage of Peroxiredoxin‐2, Alpha‐1 antitrypsin, and Haptoglobin markers in normal and cancerous tissue
FIGURE 3
FIGURE 3
Differences in the expression of Peroxiredoxin‐2 mRNA and Alpha‐1 antitrypsin mRNA and Haptoglobin markers in normal and cancerous tissues
See this image and copyright information in PMC

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