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. 2020 Mar 30;10(4):31.
doi: 10.3390/bios10040031.

Competitive HRP-Linked Colorimetric Aptasensor for the Detection of Fumonisin B1 in Food based on Dual Biotin-Streptavidin Interaction

Zui Tao  1   2   3 , You Zhou  1   2   3 , Xiang Li  1   2   3 , Zhouping Wang  1   2   3   4
Affiliations

Competitive HRP-Linked Colorimetric Aptasensor for the Detection of Fumonisin B1 in Food based on Dual Biotin-Streptavidin Interaction

Zui Tao et al. Biosensors (Basel). .

Abstract

Fumonisin B1 (FB1) is the most prevalent and toxic form among fumonisin homologues which are produced by fusarium species and it contaminates various types of food products, posing serious health hazards for humans and animals. In this work, a colorimetric assay for the detection of FB1 has been developed based on competitive horseradish peroxidase (HRP)-linked aptamer and dual biotin-streptavidin interaction. In short, a biotinylated aptamer of FB1 was immobilized on the microplate by biotin-streptavidin binding; the complementary strand (csDNA) of the aptamer was ligated with HRP by biotin-streptavidin binding again to form a csDNA-HRP sensing probe, competing with FB1 to bind to the aptamer. The color change can be observed after the addition of chromogenic and stop solution, thereby realizing the visual detection of FB1. Under optimal conditions, good linearity was observed within the concentration range of 0.5 to 300 ng/mL, with a detection of limit of 0.3 ng/mL. This assay is further validated by spike recovery tests towards beer and corn samples, it provides a simple, sensitive and reliable method for the screening of FB1 in food samples and may be potentially used as an alternative to conventional assays.

Keywords: aptamer; food safety; fumonisin B1; horseradish peroxidase; mycotoxin.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Principle of the detection of Fumonisin B1 (FB1) by the aptasensor.
Figure 2
Figure 2
(A) Optimization for the concentration of streptavidin. (B) Optimization for the concentration of BSA. (C) Optimization for the concentration of FB1 aptamer. (D) Optimization for the dilution rate of streptavidin–horseradish peroxidase (HRP).
Figure 3
Figure 3
The linear relationship between FB1 concentration and the absorbance value at 450 nm.
Figure 4
Figure 4
The evaluation for specificity of the proposed method.
Figure 5
Figure 5
The evaluation for stability of the proposed method.
See this image and copyright information in PMC

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